User:Robwarden/Notebook/Caspase-3 Activatable Probe/2009/09/10: Difference between revisions

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*1% Agarose, run for 30 min at 120V
*1% Agarose, run for 30 min at 120V


{|
|
{| border="1" style="text-align:center"
{| border="1" style="text-align:center"
|+ Gel 1
|+ Gel 1
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{| border="1" style="text-align:center"
{| border="1" style="text-align:center"
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!Sample
!Sample
| || - || 1kb || 11 || 12 || 13 || 14 || 15 || 16 || 17 || 18 ||  100bp || || ||
| || - || 1kb || 11 || 12 || 13 || 14 || 15 || 16 || 17 || 18 ||  100bp || || ||
|}
|}
|}


Revision as of 11:17, 10 September 2009

Caspase-3 Activatable Probe <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Colony Screening

Miniprep

with Qiagen kit

Digestion

  • 20 μL reaction volume
  • NEB Buffer 4
  • .5 μL of EcoRI
  • .5 μL of SgrAI
  • 10 μL of DNA
  • Incubate at 37°C for 1 hr

Agarose Gel

  • 1% Agarose, run for 30 min at 120V
Gel 1
Lane 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Sample - 1kb 1 2 3 4 5 6 7 8 9 10 100bp
Gel 2
Lane 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Sample - 1kb 11 12 13 14 15 16 17 18 100bp


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