User:Robwarden/Notebook/Caspase-3 Activatable Probe/2009/09/14: Difference between revisions

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(Autocreate 2009/09/14 Entry for User:Robwarden/Notebook/Caspase-3_Activatable_Probe)
 
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==Entry title==
==Colony Screen==
* Insert content here...
===Miniprep===
with Qiagen Kit


===Digestion===
*EcoRI & SgrAI (.5 μL each)
*10 μL colony DNA (or 1 μg control plasmid)
*NEB Buffer 4
===Gel Electrophoresis===
{| {{table}}
|+ '''Top Wells'''
!Lane
|1 || 2 || 3 || 4 || 5 || 6 || 7 || 8 || 9 || 10 || 11 || 12 || 13 || 14 || 15
|-
!Sample
|5x Control || 1kb || R-5-1 || R-5-2 || R-5-3 || R-5-4 || R-5-5 || R-5-6 || 100 bp || G-5-1 || G-5-2 || G-5-3 || G-5-4 || G-5-5 || G-5-6
|}
{| {{table}}
|+ '''Bottom Wells'''
!Lane
| 1 || 2 || 3 || 4 || 5 || 6 || 7 || 8
|-
!Sample
|1x Control || 1kb || R-1-1 || R-1-2 || R-1-3 || R-1-4 || R-1-5 || R-1-6
|}
*Naming Scheme:
**G = hgLuc, R = hRLuc8
**1 = 1xDEVD, 5 = 5xDEVD
**Final Number = Colony Number


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Revision as of 07:14, 15 September 2009

Caspase-3 Activatable Probe <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Colony Screen

Miniprep

with Qiagen Kit

Digestion

  • EcoRI & SgrAI (.5 μL each)
  • 10 μL colony DNA (or 1 μg control plasmid)
  • NEB Buffer 4

Gel Electrophoresis

Top Wells
Lane 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Sample 5x Control 1kb R-5-1 R-5-2 R-5-3 R-5-4 R-5-5 R-5-6 100 bp G-5-1 G-5-2 G-5-3 G-5-4 G-5-5 G-5-6
Bottom Wells
Lane 1 2 3 4 5 6 7 8
Sample 1x Control 1kb R-1-1 R-1-2 R-1-3 R-1-4 R-1-5 R-1-6
  • Naming Scheme:
    • G = hgLuc, R = hRLuc8
    • 1 = 1xDEVD, 5 = 5xDEVD
    • Final Number = Colony Number


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