User:Ryan T. Willett

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(Research interests)
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* ''in utero'' electroporation of DNA into rat embryos
* ''in utero'' electroporation of DNA into rat embryos
*pseudotyped recombinant retrovirus production and purification
*pseudotyped recombinant retrovirus production and purification
==Research interests==
==Research interests==

Revision as of 22:14, 28 June 2012

Ryan T. Willett
Ryan T. Willett

I am a starting up my postdoc in the laboratory of Alexandra Joyner at MSKCC. Our lab studies how the Engrailed family of homeodomain transcription factors and Sonic Hedgehog (SHH) signaling pathway shape the morphology, cytoarchitecture, cell fate specification, and circuitry formation of the cerebellum. Additionally, we study role of dysregulation of the Hedgehog pathway as a driving influence in several cancers, such as prostate and medulloblastoma. My project, in its beginning stages, will focus on characterization of the function of the Engrailed family of homeodomain transcription factors, the communication between Engrailed proteins and the Hedgehog pathway, identification of Engrailed gene targets that they act upon during transcriptional regulation, and identification of protein cofactors that they bind with to carry out this function.

OpenWetWare caught my attention in a writeup on DIY, howebrew biology in New Scientist magazine.


Contact Info

  • Laboratory of Alexandra Joyner
  • Sloan-Kettering Institute - Developmental Biology Program
  • New York, NY, USA
  • Contact Information


  • 2010 PhD, Columbia University
  • 2002 BS, Brandeis University - Biology
  • 2002 BA, Brandeis University - Biochemistry


  • Ruth L. Kirschstein National Research Service Award (F32, NINDS)
  • Brian F. Hoffman Award for Excellence in Graduate Studies of Pharmacology (Columbia University)
  • Graduated with High Honors in Biology for the undergraduate thesis "Posttranscriptional Analysis of Circadian Rhythms" in the laboratory of Michael Rosbash (Brandeis University)


  • molecular biology
  • RNA/DNA/protein biochemistry
  • cell culture and generation of stably transfected cell lines
  • dissection and primary cell culture
  • rodent survival surgery
  • mouse genetics
  • in utero electroporation of DNA into rat embryos
  • pseudotyped recombinant retrovirus production and purification
  • immunohistology

Research interests

  1. Neurodevelopment
  2. Stem cell biology
  3. Cancer biology
  4. Molecular Genetics
  5. Biochemistry
  6. Genomics
  7. Bioinformatics


Biswas SC, Zhang Y, Iyirhiaro G, Willett RT, Rodriguez-Gonzalez Y, Cregan SP, Slack RS, Park DS, Greene LA. (2010) "SERTAD1 Plays an Essential Role in Developmental and Pathological Neuron Death". J Neurosci 30(11):3973-3982

Malagelada C, Lopez-Toledano MA, Willett RT, Jin ZH, Shelanski ML, Greene LA. (2011) Role of RTP801 in Neural Stem Cell Maintenance and Differentiation. J Neurosci 31(9):3186-96

Willett RT and Greene LA. (2011) "Gata2 is Required for Migration and Differentiation of Retinorecipient Neurons in the Superior Colliculus". J Neurosci 31(12):4444-55

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