User:S Sibert/Notebook/SBB13Ntbk-Stephanie J Sibert

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==4/19/2012==
 
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sequence confirmed<br>
 
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golden gate<br>
 
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Protocol:<br>
 
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6.5ul ddH2o<br>
 
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1ul ligase buffer<br>
 
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0.5ul ligase<br>
 
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0.5ul BsMb1<br>
 
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1ul of synthon mic<br>
 
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o.5ul vector<br>
 
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==4/23/13==
 
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Transform<br>
 
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1 tube<br>
 
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20ul KCM<br>
 
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no h20<br>
 
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Rescue <br>
 
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plate<br>
 
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==4/25/13==
 
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~miniprep~<br>
 
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6.5ul H20<br>
 
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2ul Plasmid<br>
 
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1ul NEB2<br>
 
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0.5ul Bsa1<br>
 
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<Br>
 
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~map~<br>
 
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lanes 7,8,9 <br>
 
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A,B,D respectivly<br>
 
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~seq~<br>
 
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