User:S Sibert/Notebook/SBB13Ntbk-Stephanie J Sibert

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==4/16/13==
 
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Starting with <br>
 
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50ul rxn<br>
 
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31.5 ul H20<br>
 
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5ul dNTP<br>
 
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10ul Phusion buffer<br>
 
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1ul template (sample from 3/12/13)<br>
 
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1ul 10mm oligo 1 (CCOMT2-3 diluted)<br>
 
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1ul 10mm oligo 2 (CCOMT2-4 diluted)<br>
 
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0.5ul Phusion<br>
 
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Tube placed in thermocycler labeled ccomt2 4/16/13
 
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Golden Gate Set up:<br>
 
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(n)ul ddH2O to 10ul<br>
 
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1ul Ligase Buffer<br>
 
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0.5ul Ligase<br>
 
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0.5ul BsmB1<br>
 
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1ul PER CONSTRUCT<br>
 
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0.5ul vertor<br>
 
==4/18/2013==
==4/18/2013==

Revision as of 17:19, 1 December 2013

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4/18/2013

sample processed by jc anderson to miniprep step
miniprep

4/19/2012

sequence confirmed
golden gate
Protocol:
6.5ul ddH2o
1ul ligase buffer
0.5ul ligase
0.5ul BsMb1
1ul of synthon mic
o.5ul vector

4/23/13

Transform
1 tube
20ul KCM
no h20
Rescue
plate

4/25/13

~miniprep~
6.5ul H20
2ul Plasmid
1ul NEB2
0.5ul Bsa1

~map~
lanes 7,8,9
A,B,D respectivly



~seq~

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