User:S Sibert/Notebook/SBB13Ntbk-Stephanie J Sibert

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==4/19/2012==
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sequence confirmed<br>
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golden gate<br>
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Protocol:<br>
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6.5ul ddH2o<br>
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1ul ligase buffer<br>
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0.5ul ligase<br>
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0.5ul BsMb1<br>
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1ul of synthon mic<br>
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o.5ul vector<br>
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==4/23/13==
==4/23/13==

Revision as of 17:19, 1 December 2013

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4/23/13

Transform
1 tube
20ul KCM
no h20
Rescue
plate

4/25/13

~miniprep~
6.5ul H20
2ul Plasmid
1ul NEB2
0.5ul Bsa1

~map~
lanes 7,8,9
A,B,D respectivly



~seq~

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