User:S Sibert/Notebook/SBB13Ntbk-Stephanie J Sibert
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Ligations 6.5uL ddH2O 1uL Ligation buffer 1uL vector 1uL insert 0.5uL enzyme (last)
Comp Cells 1 tube cells 30uL KCM (no ddH2O)
no colonies in new plate, 3 colonies in old plate will be used to proceed.
picked colonies. by ejecting pipet tip into tube.
4) No results.
Golden Gate Set up:
sample processed by jc anderson to miniprep step