User:Samiye Yaman/Notebook/Lab 571/2011/09/21: Difference between revisions
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==Description== | ==Description== | ||
Procedures from the day before was continued | |||
1)A culture media of E.Coli was prepared for the protein expression. | |||
2)GFP containing a cysteine was prepared by using PCR. | |||
-The solution with the wax was was removed from thermocycler. | |||
-By using pipette, the wax was removed from the solution. | |||
-By suing 0.25g of agarose and 25 mL of TAE buffer, 0.01g/mL agarose gel was prepared. There were particles floating in the solution | |||
-The agarose gel was placed into the microwave for 40 seconds in order to have a homogeneous solution. | |||
-The clear homogeneous solution was placed into a mold for 15 min to cool down and solidify. | |||
- More TAE buffer was poured to the top of the agarose gel when it became solid. | |||
-1 ul of blue dye and glycerol mixture and 5uL of PCR were mixed well before loading them into the gel. | |||
-80 V was passed through the gel for 40 min. | |||
==Data== | ==Data== | ||
Revision as of 11:54, 26 September 2011
 Biomaterials Design Lab
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Entry titleObjectiveDescriptionProcedures from the day before was continued 1)A culture media of E.Coli was prepared for the protein expression. 2)GFP containing a cysteine was prepared by using PCR. -The solution with the wax was was removed from thermocycler. -By using pipette, the wax was removed from the solution. -By suing 0.25g of agarose and 25 mL of TAE buffer, 0.01g/mL agarose gel was prepared. There were particles floating in the solution -The agarose gel was placed into the microwave for 40 seconds in order to have a homogeneous solution. -The clear homogeneous solution was placed into a mold for 15 min to cool down and solidify. - More TAE buffer was poured to the top of the agarose gel when it became solid. -1 ul of blue dye and glycerol mixture and 5uL of PCR were mixed well before loading them into the gel. -80 V was passed through the gel for 40 min. Data
NotesThis area is for any observations or conclusions that you would like to note.
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