User:Samiye Yaman/Notebook/Lab 571/2011/10/05
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Entry titleObjectiveDescriptionDataThe graph shows the intensity of Ru(bpy)(phen) in standard solutions and wavelength. The standard solution with the highest concentration of Ru(bpy)(phen) had the highest intensity whereas the standard solution with the lowest concentration had the lowest intensity. The graph shows the integrated density and concentration of Ru(bpy)(phen) in standard solutions. The graph has a positive slope of 39.68. Integrated density is at the highest when the concentration of the Ru(bpy)(phen) in standard solutions was greatest. Determining the Fluorescence of MBP Identification of each lined on the graph Navy Blue= Solution A & Red= Solution B & Green= Solution C & Purple= Solution D & Light Blue= Solution D Corrected Intensity vs Wavelength (nm) The graph shows the relationship between the corrected intensity and wavelength. Solution B had the highest corrected intensity whereas Solution D had the lowest corrected intensity. The intensity values for some of the measurements were higher than it supposed to be therefore the Fluorimeter couldn’t read. For Solution A Corrected Intensity/ Concentration (uM) vs Wavelength (nm) The graph shows the relationship between the corrected intensity/concentration and the wavelength measured for the solution A. After 430 nm, the corrected intensity/ concentration started to decrease with increasing wavelength. For Solution B Corrected Intensity/ Concentration (uM) vs Wavelength (nm) The graph shows the relationship between the corrected intensity/concentration and the wavelength measured for the solution B. The graph had a positive slope from 490 uM to 2000uM and negative slope from 2000 uM until it reaches zero.
The graph shows the relationship between the corrected intensity/concentration and the wavelength measured for the solution C. The graph had a positive slope until the ratio reaches around 9800 uM then the slope started to become negative. Highest corrected intensity was measured around 360 nm.
For Solution E Corrected Intensity/ Concentration (uM) vs Wavelength (nm) The graph shows the relationship between the corrected intensity/Concentration and the wavelength measured for the solution E. The graph has many fluctuations. Highest corrected intensity/ concentration ratio was measured around 360 nm. NotesThis area is for any observations or conclusions that you would like to note.
