User:Samiye Yaman/Notebook/Lab 571/2011/11/01: Difference between revisions
Samiye Yaman (talk | contribs) |
Samiye Yaman (talk | contribs) (→Data) |
||
Line 42: | Line 42: | ||
Reverse: 3' ATG CTG CTA CTG CTA TTC ACA GCT ACC CCT AGG CTT AAG 5' | Reverse: 3' ATG CTG CTA CTG CTA TTC ACA GCT ACC CCT AGG CTT AAG 5' | ||
Synthesizing Goldnanoparticles | |||
t=0 min | |||
-Test tube A: yellowish color | |||
-Test tube B: clear color | |||
t:30 min | |||
-Test tube A: yellow cloud at the top of the solution, bubbles in and out of the yellow cloud | |||
-Test tube B: clear solution | |||
==Notes== | ==Notes== |
Revision as of 10:32, 1 November 2011
Biomaterials Design Lab | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Entry titleObjectiveDescriptionPCR ---Online webpage,( http://www.basic.northwestern.edu/biotools/oligocalc.html) was used to have a better understanding of PCR and find the forward and reverse primers necessary for the experiment.
Reverse: 3' ATG CTG CTA CTG CTA TTC ACA GCT ACC CCT AGG CTT AAG 5' ---A solution of 5 uL of 10X Pfu reaction buffer,1 uL of PfuTurbo DNA polymerase,1 uL of dNTP mix,1 uL of template DNA, 1.25 uL of primer 1 and 1.25 uL of primer 2 was prepared. ---Before placing the solution in the thermocycler at 95°C for 30 seconds, 25 uL of BioRad Chill out wax, dark pink, was added to the top of the solution in order to prevent the evaporation of the solution. ---The solution with the wax was kept in the thermocycler, which was at 4°C, for 24 hours. Synthesizing gold nanoparticles. -Two test tubes were prepared. -Test tube A had 1 ml of HCl, 1 ml of BSA, and 8 ml of water.(with this order) -Test tube B had 1 ml of BSA,7 ml of water and 2 ml of chloroauric acid.(with this order) -Both test tubes were placed in to the oven which was at 80 degree celcius and took it out of the oven for 10 min every 30 minutes. DataPCR Forward: 5' TAC GAC GAT GAC GAT AAG TGT CGA TGG GGA TCC GAA TTC 3' Reverse: 3' ATG CTG CTA CTG CTA TTC ACA GCT ACC CCT AGG CTT AAG 5'
t=0 min -Test tube A: yellowish color -Test tube B: clear color
-Test tube A: yellow cloud at the top of the solution, bubbles in and out of the yellow cloud -Test tube B: clear solution NotesThis area is for any observations or conclusions that you would like to note.
|