User:Samiye Yaman/Notebook/Lab 571/2011/11/01
 Biomaterials Design Lab
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Entry titleObjectiveDescriptionPCR ---Online webpage,( http://www.basic.northwestern.edu/biotools/oligocalc.html) was used to have a better understanding of PCR and find the forward and reverse primers necessary for the experiment.
Reverse: 3' ATG CTG CTA CTG CTA TTC ACA GCT ACC CCT AGG CTT AAG 5' ---A solution of 5 uL of 10X Pfu reaction buffer,1 uL of PfuTurbo DNA polymerase,1 uL of dNTP mix,1 uL of template DNA, 1.25 uL of primer 1 and 1.25 uL of primer 2 was prepared. ---Before placing the solution in the thermocycler at 95°C for 30 seconds, 25 uL of BioRad Chill out wax, dark pink, was added to the top of the solution in order to prevent the evaporation of the solution. ---The solution with the wax was kept in the thermocycler, which was at 4°C, for 24 hours. Synthesizing gold nanoparticles. -Two test tubes were prepared.
-Test tube A had 1 ml of HCl, 1 ml of BSA, and 8 ml of water.(with this order)
-Test tube B had 1 ml of BSA,7 ml of water and 2 ml of chloroauric acid.(with this order)
-Both test tubes were placed in to the oven which was at 80 degree celcius and took it out of the oven
for 15 min every 30 minutes.
DataPCR Forward: 5' TAC GAC GAT GAC GAT AAG TGT CGA TGG GGA TCC GAA TTC 3' Reverse: 3' ATG CTG CTA CTG CTA TTC ACA GCT ACC CCT AGG CTT AAG 5'
t=0 min -Test tube A: clear color -Test tube B: yellowish color
-Test tube A: clear solution -Test tube B: yellow cloud at the top of the solution, bubbles in and out of the yellow cloud t:60 min -Test tube A: clear solution -Test tube B: formation of black solid t:120 -Test tube A:clear solution -Test tube B: formation of black solid t:150min -Test tube A:clear solution -Test tube B: formation of the black solid NotesThis area is for any observations or conclusions that you would like to note.
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