User:Samiye Yaman/Notebook/Lab 571/2011/11/02: Difference between revisions
Samiye Yaman (talk | contribs) |
Samiye Yaman (talk | contribs) (→Data) |
||
| Line 54: | Line 54: | ||
t=90min :formation of a greenish yellow solid. The bubbles disappeared. | t=90min :formation of a greenish yellow solid. The bubbles disappeared. | ||
t=120min : the greenish yellow solid shrank more and it is splinted in to two pieces. | |||
==Notes== | ==Notes== | ||
Revision as of 12:43, 2 November 2011
 Biomaterials Design Lab
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Entry titleObjectiveDescription3 different procedures were made. 1)Synthesizing gold nanoparticles. -A test tube with 1 ml of BSA,7 ml of water and 2 ml of chloroauric acid.(with this order) was prepared -The test tube was placed in to the oven which was at 80 degree celcius and took it out of the oven for 15 min every 30 minutes. 2)PCR continued - 10ul of DNA and 2ul of the loading buffer was placed in to a PCR tubes. -10 ul of the sample was loaded to the gel and was run.
3) Preparing Agar Plate - 0.875 g LB, 0.7g agar and 35ml of H2O were added to the 200 ml flask. -The flask was autoclaved. -All the agar solution was placed in to a petri dish with addition of 35ul of antibiotics. -DNA was transferred into cells. - The DNA was placed in to ice for 15min. - 35 ul of the cells and 5 ul of DNA were combined and incubated on ice for 30min. -Then it is placed heat shocked at 42C for 30 sec. - Incubated on ice for 5 min. The DNA/cell mixture was then heat shocked at 42C for 30 sec, and subsequently incubated on ice for 5 min. 250μL of SOC media was added to the mixture, which was then incubated in the shaker at 37C for 1 hr. 100μL of cells were spread (using sterile techniques) on the LB/agar plate. The plate was then inverted and stored in a 37C oven overnight. Data
t=0min : clear solution t=30 min : formation of bubbles and formation of the yellow cloud t=60min :the size of the yellow cloud shrink and it has a darker greenish yellow color. The bubbles are still present however there are less of them. t=90min :formation of a greenish yellow solid. The bubbles disappeared. t=120min : the greenish yellow solid shrank more and it is splinted in to two pieces. NotesThis area is for any observations or conclusions that you would like to note.
| |
