User:Samiye Yaman/Notebook/Lab 571/2011/11/02: Difference between revisions
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1)Synthesizing gold nanoparticles. | 1)Synthesizing gold nanoparticles. | ||
-A test tube with 1 ml of BSA,7 ml of water and 2 ml of chloroauric acid.(with this order) was prepared | |||
-The test tube was placed in to the oven which was at 80 degree celcius and took it out of the oven for 15 min every 30 minutes. | |||
2)PCR continued | 2)PCR continued | ||
| Line 33: | Line 32: | ||
- 0.875 g LB, 0.7g agar and 35ml of H2O were added to the 200 ml flask. | - 0.875 g LB, 0.7g agar and 35ml of H2O were added to the 200 ml flask. | ||
-The flask was autoclaved. | -The flask was autoclaved. | ||
- | -All the agar solution was placed in to a petri dish with addition of 35ul of antibiotics. | ||
-DNA was transferred into cells. | |||
- The DNA was placed in to ice for 15min. | |||
- 35 ul of the cells and 5 ul of DNA were combined and incubated on ice for 30min. | |||
- Then it is placed heat shocked at 42C for 30 sec. | |||
- Incubated on ice for 5 min. | |||
- 250ul of SOC media was added. | |||
-incubated for an hour at 37C. | |||
-100uL of cells were spread on Lb/agar plate. | |||
-The plate was then inverted and stored in a 37C oven overnight. | |||
==Data== | ==Data== | ||
Synthesizing Gold nanoparticles | |||
t=0min : clear solution | |||
t=30 min : formation of bubbles and formation of the yellow cloud | |||
t=60min :the size of the yellow cloud shrink and it has a darker greenish yellow color. The bubbles are still present however there are less of them. | |||
t=90min :formation of a greenish yellow solid. The bubbles disappeared. | |||
t=120min : the greenish yellow solid shrank more and it is splinted in to two pieces. | |||
t=150min: same as at 120min | |||
LB agar plate | |||
-No growth of cells | |||
==Notes== | ==Notes== | ||
Revision as of 11:41, 9 December 2011
 Biomaterials Design Lab
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Entry titleObjectiveDescription3 different procedures were made. 1)Synthesizing gold nanoparticles. -A test tube with 1 ml of BSA,7 ml of water and 2 ml of chloroauric acid.(with this order) was prepared -The test tube was placed in to the oven which was at 80 degree celcius and took it out of the oven for 15 min every 30 minutes. 2)PCR continued - 10ul of DNA and 2ul of the loading buffer was placed in to a PCR tubes. -10 ul of the sample was loaded to the gel and was run.
3) Preparing Agar Plate - 0.875 g LB, 0.7g agar and 35ml of H2O were added to the 200 ml flask. -The flask was autoclaved. -All the agar solution was placed in to a petri dish with addition of 35ul of antibiotics. -DNA was transferred into cells. - The DNA was placed in to ice for 15min. - 35 ul of the cells and 5 ul of DNA were combined and incubated on ice for 30min. - Then it is placed heat shocked at 42C for 30 sec. - Incubated on ice for 5 min. - 250ul of SOC media was added. -incubated for an hour at 37C. -100uL of cells were spread on Lb/agar plate. -The plate was then inverted and stored in a 37C oven overnight. DataSynthesizing Gold nanoparticles t=0min : clear solution t=30 min : formation of bubbles and formation of the yellow cloud t=60min :the size of the yellow cloud shrink and it has a darker greenish yellow color. The bubbles are still present however there are less of them. t=90min :formation of a greenish yellow solid. The bubbles disappeared. t=120min : the greenish yellow solid shrank more and it is splinted in to two pieces. t=150min: same as at 120min LB agar plate -No growth of cells NotesThis area is for any observations or conclusions that you would like to note.
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