User:Samiye Yaman/Notebook/Lab 571/2011/11/08

From OpenWetWare
< User:Samiye Yaman‎ | Notebook‎ | Lab 571‎ | 2011‎ | 11
Revision as of 11:44, 9 December 2011 by Samiye Yaman (talk | contribs) (→‎Data)
Jump to navigationJump to search
Biomaterials Design Lab <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Entry title

Objective

Description

1)Synthesizing gold nanoparticles.(Concentration of BSA=1.55 uM. Concentration of HAuCl4=0.29 mM)

-Epi Test tube A with 100 ul of BSA, 800ul of water and 100ul of chloroauric acid.(with this order) was prepared. The test tube was placed in to the heat block which was at 75 degree celcius and took it out of the oven for 10 min every 30 minutes.

-Epi Test tube B with 100ul of BSA, 800ul of water and 100ulof chloroauric acid.The test tube was placed in to the heat block which was at 75 degree celcius.

2)Mini prep

- 5 ml of LB, a cell sample taken with a tooth pick and 5ul of ampicilin were placed in to a test tube.

Data

t=30mins

Test Tube A: formation of bubbles and grey fibers.

t=60mins

Test Tube A: formation of grey fibers.

t=90mins

Test Tube A: clear solution, dark grey material.

t=120mins

Test tube A: clear solution, dark grey material.

Test tube B: light purple solution.

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.