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==Objective== | ==Objective== | ||
Synthesizing nanoparticles and testing the effects of different solution on the fibers | |||
==Description== | ==Description== | ||
'''Part 1''' | |||
Synthesizing nanoparticles | |||
-1ml of BSA, 0.5 mL of HAuCl and 8.5 ml of water was placed in to a test tube. | |||
-The test tube was placed in to the oven which was at 70degree celcius for 3 hours. (Concentration of BSA is 30uM and HAuCl is 4.17uM.) | |||
-epi tube 2-1 from 15/11/2011 placed in to the heat block again. | |||
-Epi test tube 1 was prepared. 100ul BSA, 50uL HAuCl4, 850 ul water | |||
-Epi test tube 2 was prepared. 25 uL HAuCl4,50uL BSA, 925 uL water. (with this order) | |||
-Epi test tube 3 was prepared. 50uL BSA, 25 uL HAuCl4, 925 uL water.(with this order) | |||
'''Part 2''' | |||
Testing the effects of different solution on the fibers. Fibers are added to 4 different solutions. | |||
-Solution 1 had 1 ml of 1M Tris | |||
-Solution 2 had 1 ml of 6M of NaOH | |||
-Solution 3 had 1 ml DMSO. | |||
-Solution 4 had 1 ml DMSO. | |||
==Data== | ==Data== | ||
'''Part A''' | |||
The large reaction tube= a dark purple color. | |||
Epitube 1:medium pinkish purple color. | |||
Epitube 2:light pinkish purple color | |||
Epitube 3: light pinkish purple color. | |||
'''Part B''' | |||
Solution 1 1: fibers did not dissolve. | |||
Solution 2: fibers did not dissolve spontaneously however, fibers dissolved after one week. | |||
Solution 3: fibers dissolved spontaneously. a week later, most of the fibers were dissolved and dark purple solution was left. | |||
==Notes== | ==Notes== | ||
Revision as of 11:55, 9 December 2011
 Biomaterials Design Lab
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Entry titleObjectiveSynthesizing nanoparticles and testing the effects of different solution on the fibers DescriptionPart 1 Synthesizing nanoparticles -1ml of BSA, 0.5 mL of HAuCl and 8.5 ml of water was placed in to a test tube. -The test tube was placed in to the oven which was at 70degree celcius for 3 hours. (Concentration of BSA is 30uM and HAuCl is 4.17uM.) -epi tube 2-1 from 15/11/2011 placed in to the heat block again. -Epi test tube 1 was prepared. 100ul BSA, 50uL HAuCl4, 850 ul water -Epi test tube 2 was prepared. 25 uL HAuCl4,50uL BSA, 925 uL water. (with this order) -Epi test tube 3 was prepared. 50uL BSA, 25 uL HAuCl4, 925 uL water.(with this order) Part 2 Testing the effects of different solution on the fibers. Fibers are added to 4 different solutions. -Solution 1 had 1 ml of 1M Tris -Solution 2 had 1 ml of 6M of NaOH -Solution 3 had 1 ml DMSO. -Solution 4 had 1 ml DMSO. DataPart A The large reaction tube= a dark purple color. Epitube 1:medium pinkish purple color. Epitube 2:light pinkish purple color Epitube 3: light pinkish purple color. Part B Solution 1 1: fibers did not dissolve. Solution 2: fibers did not dissolve spontaneously however, fibers dissolved after one week. Solution 3: fibers dissolved spontaneously. a week later, most of the fibers were dissolved and dark purple solution was left. NotesThis area is for any observations or conclusions that you would like to note.
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