User:Samiye Yaman/Notebook/Lab 571/2012/02/15: Difference between revisions
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== '''Determining Processes of Isolating Gold Nanoparticles Using Refolding Buffers''' == | |||
==Objective== | |||
-Synthesizing AuNP solutions. (purple solution) | |||
-UV-Vis of Solution 1 (Au/BSA mole ratio = 70) synthesized on 31/01/2012 | |||
[[http://openwetware.org/wiki/User:Samiye_Yaman/Notebook/Lab_571/2012/01/31]] | |||
-Determining the effect of tris on solution 2 (Au/BSA mole ratio = 166) synthesized on 31/01/2012 | |||
[[http://openwetware.org/wiki/User:Samiye_Yaman/Notebook/Lab_571/2012/01/31]] | |||
==Description== | |||
<b>Synthesizing AuNP</b> | |||
# Combine 2 mL of 3.66 mM HAuCl<sub>4</sub> and 6.04 mL of 0.0173 mM BSA in a test tube. | |||
# Dilute to 20 mL with water. | |||
# Place in oven at 80°C. | |||
# Leave solutions in oven for 3 hr. | |||
# Continue to take UV-vis data of 1 mL 70 Au/BSA ratio solution synthesized on [[User:Elaine Marie Robbins/Notebook/CHEM-496/2012/01/31| 01/31/12]] over time. | |||
<b>Determining the effect of tris buffer on 166 Au/BSA ratio solution</b> | |||
# Take UV-vis data of 166 Au/BSA ratio solution synthesized on [[User:Elaine Marie Robbins/Notebook/CHEM-496/2012/01/31| 01/31/12]]. | |||
# Add 2 mL 50 mM tris buffer (pH 10.1) to solid protein fibers (no solution). | |||
# Allow fibers to settle. | |||
# Take UV-vis data of tris/fiber solution. | |||
==Data== | |||
[[Image:Aunp_solution.JPG]] |
Revision as of 21:17, 24 March 2012
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Determining Processes of Isolating Gold Nanoparticles Using Refolding BuffersObjective-Synthesizing AuNP solutions. (purple solution) -UV-Vis of Solution 1 (Au/BSA mole ratio = 70) synthesized on 31/01/2012 [[1]] -Determining the effect of tris on solution 2 (Au/BSA mole ratio = 166) synthesized on 31/01/2012 [[2]]
DescriptionSynthesizing AuNP
Data |