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== '''Statement of Work''' ==
== '''Determining Processes of Isolating Gold Nanoparticles Using Refolding Buffers''' ==


==Description==
'''Part A'''


The proposed project will address the following four topics:
Syntsizing purple fibers (Au/BSA mole ratio = 166)


1. Determine the process by which refolding buffers affect the nanoparticle aggregate using UV/VIS spectroscopy.  
*Combine 1 mL of 3.66 mM HAuCl4 and 1.27 mL of 0.0173 mM BSA in a test tube.


2. Test methods of removing gold nanoparticles from BSA protein while keeping them in solution.  
*Dilute to 10 mL with water.


3. Determine optimum methods of adding nanoparticle solution to refolding buffer.
*Place in oven. (75°C)


4. Compare various refolding buffer options to determine the most stable solution using UV/VIS spectroscopy.
*Leave the test tube in oven for 3 hrs.
 
'''Part B'''
 
Determining the effect of tris buffer on 166 Au/BSA ratio solution
 
*Take UV-vis data of 166 Au/BSA ratio solution synthesized on 01/31/12 [[http://openwetware.org/wiki/User:Samiye_Yaman/Notebook/Lab_571/2012/01/31]] and modified on [[http://openwetware.org/wiki/User:Samiye_Yaman/Notebook/Lab_571/2012/02/01]]
 
*Centrifuge for 10 min at 13,200 rpm.
 
*Take UV-vis data of supernatant.
 
==Data==
[[Image:Fibers.png]]
 
 
[[Image:Ionic_strenght21.02.png]]
 
[[Image:Week1.week2..png]]

Revision as of 21:05, 25 March 2012

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Determining Processes of Isolating Gold Nanoparticles Using Refolding Buffers

Description

Part A

Syntsizing purple fibers (Au/BSA mole ratio = 166)

  • Combine 1 mL of 3.66 mM HAuCl4 and 1.27 mL of 0.0173 mM BSA in a test tube.
  • Dilute to 10 mL with water.
  • Place in oven. (75°C)
  • Leave the test tube in oven for 3 hrs.

Part B

Determining the effect of tris buffer on 166 Au/BSA ratio solution

  • Take UV-vis data of 166 Au/BSA ratio solution synthesized on 01/31/12 [[1]] and modified on [[2]]
  • Centrifuge for 10 min at 13,200 rpm.
  • Take UV-vis data of supernatant.

Data
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