User:Samiye Yaman/Notebook/Lab 571/2012/02/21: Difference between revisions

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== '''Statement of Work''' ==
== '''Determining Processes of Isolating Gold Nanoparticles Using Refolding Buffers''' ==


==Description==
'''Part A'''
Syntsizing 2 solutions of AuNP with different ratio:


The proposed project will address the following four topics:
---Solution 1 (Au/BSA mole ratio = 70)


1. Determine the process by which refolding buffers affect the nanoparticle aggregate using UV/VIS spectroscopy.  
*Combine 1 mL of 3.66 mM HAuCl4 and 3.02 mL of 0.0173 mM BSA in a test tube.


2. Test methods of removing gold nanoparticles from BSA protein while keeping them in solution.  
*Dilute to 10 mL with water.


3. Determine optimum methods of adding nanoparticle solution to refolding buffer.
*Place in oven.(80°C)


4. Compare various refolding buffer options to determine the most stable solution using UV/VIS spectroscopy.
---Solution 2 (Au/BSA mole ratio = 166)
 
*Combine 1 mL of 3.66 mM HAuCl4 and 1.27 mL of 0.0173 mM BSA in a test tube.
 
*Dilute to 10 mL with water.
 
*Place in oven. (80°C)
 
'''Part B'''
Leave solutions in oven for 2.5 hr.

Revision as of 12:59, 29 February 2012

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Determining Processes of Isolating Gold Nanoparticles Using Refolding Buffers

Description

Part A Syntsizing 2 solutions of AuNP with different ratio:

---Solution 1 (Au/BSA mole ratio = 70)

  • Combine 1 mL of 3.66 mM HAuCl4 and 3.02 mL of 0.0173 mM BSA in a test tube.
  • Dilute to 10 mL with water.
  • Place in oven.(80°C)

---Solution 2 (Au/BSA mole ratio = 166)

  • Combine 1 mL of 3.66 mM HAuCl4 and 1.27 mL of 0.0173 mM BSA in a test tube.
  • Dilute to 10 mL with water.
  • Place in oven. (80°C)

Part B Leave solutions in oven for 2.5 hr.

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