User:Samiye Yaman/Notebook/Lab 571/2012/04/04: Difference between revisions

From OpenWetWare
< User:Samiye Yaman‎ | Notebook‎ | Lab 571‎ | 2012‎ | 04
Jump to navigationJump to search
Line 18: Line 18:


==='''Description'''===
==='''Description'''===
-Add 40 μL of BSA or AuNP solution (synthesized on 02/15/12)to 40 μL 4 M NaOH.
-Add 2μL 450 mM DTT.
-Heat in microwave for 1 min.
-Take UV-vis data.


==='''Data'''===
==='''Data'''===

Revision as of 05:08, 17 April 2012

Biomaterials Design Lab <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>




Objective

Testing the effects of microwave-assisted base hydrolysis in order to determine the concentration of free BSA in solution.

Description

-Add 40 μL of BSA or AuNP solution (synthesized on 02/15/12)to 40 μL 4 M NaOH.

-Add 2μL 450 mM DTT.

-Heat in microwave for 1 min.

-Take UV-vis data.

Data