User:Samiye Yaman/Notebook/Lab 581/2012/10/17

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Objective

Description

Part 1 (ISE)

  • 0.1 ml of Sodium Ionic Strength Adjuster and 3 ml of each Sodium Sulfate solution was mixed in separate test tubes.
  • Channel 1 of the Orien Versa Star was used.
  • Before each measurement 30 sec was wait.


Part 2 Preparation of Porphyrin dilutions

  • Dilution 1: 1 ml of stock solution and 9 ml of HPLC water (concentration is 100 micM)
  • Dilution 2: 5 ml of dilution 1 and 5 ml of HPLC water (concentration is 50 micM)
  • Dilution 3: 5 ml of dilution 2 and 5 ml of HPLC water (concentration is 25 micM)
  • Dilution 4: 5 ml of dilution 3 and 5 ml of HPLC water (concentration is 12.5 micM)
  • Dilution 5: 5 ml of dilution 4 and 5 ml of HPLC water (concentration is 6.25 micM)

Data

  • Add data and results here...

Notes

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