User:Samiye Yaman/Notebook/Lab 581/2012/10/24: Difference between revisions

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==Description==
==Description==


'''Part 1''' '''(ISE)'''
'''Part 1''' '''Preparation of porphyrin films'''


*0.1 ml of Sodium Ionic Strength Adjuster and 3 ml of each Sodium Sulfate solution was mixed in separate test tubes.
* Add 2 drops of HCL, 1 ml of gluteraldehyde and 1 ml of porphyrin after the solution is homogeneous and cooled down.


*Channel 1 of the Orien Versa Star was used.
*Keep everything under the hood.


*Before each measurement 30 sec was wait.
'''Part 2''' '''UV-VIS of porphyrin dilutions'''


'''Part 3''' '''Prepare dilutions of 50micM Na2SO4, 50micM H2SO4'''


'''Part 2''' '''Preparation of Porphyrin dilutions'''
'''Na2SO4'''


*Dilution 1: 1 ml of stock solution and 9 ml of HPLC water (concentration is 100 micM)
*FW: 142.07 g/ml


*Dilution 2: 5 ml of dilution 1 and 5 ml of HPLC water (concentration is 50 micM)
*1/142.07 g/mol x 50micM/L x 10^(-6)mol/1micM x 1/1000ml x 10ml= 0.071035 g in 10 ml of HPLC water.


*Dilution 3: 5 ml of dilution 2 and 5 ml of HPLC water (concentration is 25 micM)
*(50micL) (x) = (50micM) (10mL)
 
         
*Dilution 4: 5 ml of dilution 3 and 5 ml of HPLC water (concentration is 12.5 micM)
          x  = 0.01 ml =10micL of Na2SO4 in 9.99ml HPLC water
 
*Dilution 5: 5 ml of dilution 4 and 5 ml of HPLC water (concentration is 6.25 micM)


==Data==
==Data==

Revision as of 20:46, 30 October 2012

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Objective

Description

Part 1 Preparation of porphyrin films

  • Add 2 drops of HCL, 1 ml of gluteraldehyde and 1 ml of porphyrin after the solution is homogeneous and cooled down.
  • Keep everything under the hood.

Part 2 UV-VIS of porphyrin dilutions

Part 3 Prepare dilutions of 50micM Na2SO4, 50micM H2SO4

Na2SO4

  • FW: 142.07 g/ml
  • 1/142.07 g/mol x 50micM/L x 10^(-6)mol/1micM x 1/1000ml x 10ml= 0.071035 g in 10 ml of HPLC water.
  • (50micL) (x) = (50micM) (10mL)
          x  = 0.01 ml =10micL of Na2SO4 in 9.99ml HPLC water

Data

ISE results for sodium sulfate dilutions

  • Dilution 1(1.25ppm)= -180mV
  • Dilution 2(2.5ppm)= -182.6mV
  • Dilution 3(5ppm) = -182.4mV
  • Dilution 4(10ppm )= -177.4mV
  • Dilution 5(20ppm)= -174.6mV


  • We decided detection limit was between dilution 1 and 2.
  • More negative number means less sodium ion in the solution.
  • The porphyrin films prepared on 10/10/2012 had a yellowish green color. They were not clear as the other films.

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



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