User:Samiye Yaman/Notebook/Lab 581/2012/10/24: Difference between revisions

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==Description==
==Description==


'''Part 1''' '''(ISE)'''
'''Part 1 : Preparation of porphyrin films'''


*0.1 ml of Sodium Ionic Strength Adjuster and 3 ml of each Sodium Sulfate solution was mixed in separate test tubes.
* Add 2 drops of HCL, 1 ml of gluteraldehyde and 1 ml of porphyrin after the solution is homogeneous and cooled down.


*Channel 1 of the Orien Versa Star was used.
*Keep everything under the hood.


*Before each measurement 30 sec was wait.
'''Part 2 : UV-VIS of porphyrin dilutions prepared on 17/10/2012'''


*'''A)''' from the UV-Vis results we concluded that it is necessary to make extra 2 dilutions as dilution 1 and 2 are out of range. They are too concentrated. We would like to obtain a peak lower than 1.


'''Part 2''' '''Preparation of Porphyrin dilutions'''
*Dilution 6: 4 ml of dilution 5 + 4 ml of HPLC water.(0.3375micM)


*Dilution 1: 1 ml of stock solution and 9 ml of HPLC water (concentration is 100 micM)
*Dilution 7: 4ml of dilution 6 + 4 ml of HPLC water. (0.16785micM)


*Dilution 2: 5 ml of dilution 1 and 5 ml of HPLC water (concentration is 50 micM)
'''Part 3 : Prepare dilutions of 50micM Na2SO4, 50micM Al2(SO4)3, 50micM Fe2(SO4)3''' (HPLC WATER IS USED)


*Dilution 3: 5 ml of dilution 2 and 5 ml of HPLC water (concentration is 25 micM)
'''Na2SO4'''


*Dilution 4: 5 ml of dilution 3 and 5 ml of HPLC water (concentration is 12.5 micM)
*FW: 142.07 g/ml


*Dilution 5: 5 ml of dilution 4 and 5 ml of HPLC water (concentration is 6.25 micM)
*1/142.07 g/mol x 50micM/L x 10^(-6)mol/1micM x 1/1000ml x 10ml= 0.071035 g in 10 ml of HPLC water.
 
*(50mM) (x) = (50micM) (10mL)
         
* x  = 0.01 ml =10micL of Na2SO4 in 9.99ml HPLC water
 
 
'''Al2(SO4)3'''
 
*FW: 456.33g/mol
 
*456.33g/mol x 50mmol/L x 10^(-3)/1mmol x 1L/1000mL x 10 mml= 0.392g in 10 mL H2O.
 
*50micM=10micL of 50mM in 999micL H2O
 
 
'''Fe2(SO4)3''' (72wt%)
 
*FW:399.88g/mol
 
* 0.72 x 399.88g/mol x 50mmol/L x 10^(-3)/1mmol x 1L/1000mL x 10 mml= 0.144g in 10 mL H2O.
 
*50micM=10micL of 50mM in 999micL H2O


==Data==
==Data==

Revision as of 08:09, 31 October 2012

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Objective

Description

Part 1 : Preparation of porphyrin films

  • Add 2 drops of HCL, 1 ml of gluteraldehyde and 1 ml of porphyrin after the solution is homogeneous and cooled down.
  • Keep everything under the hood.

Part 2 : UV-VIS of porphyrin dilutions prepared on 17/10/2012

  • A) from the UV-Vis results we concluded that it is necessary to make extra 2 dilutions as dilution 1 and 2 are out of range. They are too concentrated. We would like to obtain a peak lower than 1.
  • Dilution 6: 4 ml of dilution 5 + 4 ml of HPLC water.(0.3375micM)
  • Dilution 7: 4ml of dilution 6 + 4 ml of HPLC water. (0.16785micM)

Part 3 : Prepare dilutions of 50micM Na2SO4, 50micM Al2(SO4)3, 50micM Fe2(SO4)3 (HPLC WATER IS USED)

Na2SO4

  • FW: 142.07 g/ml
  • 1/142.07 g/mol x 50micM/L x 10^(-6)mol/1micM x 1/1000ml x 10ml= 0.071035 g in 10 ml of HPLC water.
  • (50mM) (x) = (50micM) (10mL)
  • x = 0.01 ml =10micL of Na2SO4 in 9.99ml HPLC water


Al2(SO4)3

  • FW: 456.33g/mol
  • 456.33g/mol x 50mmol/L x 10^(-3)/1mmol x 1L/1000mL x 10 mml= 0.392g in 10 mL H2O.
  • 50micM=10micL of 50mM in 999micL H2O


Fe2(SO4)3 (72wt%)

  • FW:399.88g/mol
  • 0.72 x 399.88g/mol x 50mmol/L x 10^(-3)/1mmol x 1L/1000mL x 10 mml= 0.144g in 10 mL H2O.
  • 50micM=10micL of 50mM in 999micL H2O

Data

ISE results for sodium sulfate dilutions

  • Dilution 1(1.25ppm)= -180mV
  • Dilution 2(2.5ppm)= -182.6mV
  • Dilution 3(5ppm) = -182.4mV
  • Dilution 4(10ppm )= -177.4mV
  • Dilution 5(20ppm)= -174.6mV


  • We decided detection limit was between dilution 1 and 2.
  • More negative number means less sodium ion in the solution.
  • The porphyrin films prepared on 10/10/2012 had a yellowish green color. They were not clear as the other films.

Notes

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