User:Samiye Yaman/Notebook/Lab 581/2013/01/30

(Difference between revisions)
 Revision as of 15:37, 1 February 2013 (view source) (→Data)← Previous diff Revision as of 21:19, 5 February 2013 (view source) (→Description)Next diff → Line 18: Line 18: *prepare 2 sets of each *prepare 2 sets of each - 1)0.5g of 22,000 + 6 ml water + 1)0.5g of 22,000 + 6 ml water [[User:Matt Hartings|Matt Hartings]] 0.5g of what??? 2)0.5 of 89,000-98,000 + 6 ml water 2)0.5 of 89,000-98,000 + 6 ml water Line 42: Line 42: 4)0.5g of 146,000-186,000 + 6ml of water 4)0.5g of 146,000-186,000 + 6ml of water - *add 6micL of R6G in to each hydrogels. + *add 6micL of R6G in to each hydrogels. [[User:Matt Hartings|Matt Hartings]] there is a μL symbol you can use down below *placed into -20C at noon. *placed into -20C at noon. + + [[User:Matt Hartings|Matt Hartings]] You should also add a description of your full procedure for this process here. Why are you doing these things. At some point you'll have to mention it. This (the first page of your notebook) is the best place to do it. ==Data== ==Data==

Revision as of 21:19, 5 February 2013

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Description

Part 1: Prepare hydrogels

• prepare 2 sets of each

1)0.5g of 22,000 + 6 ml water Matt Hartings 0.5g of what???

2)0.5 of 89,000-98,000 + 6 ml water

3)0.5g of 130,000 + 6 ml of water

4)0.5g of 146,000-186,000 + 6ml of water

• placed into -20C at 10:10 for 1) and 2), 3), and 4) at 10:20.

Part 2: Prepare Hydrogels with 0.5mM of R6G dye

• 0.00479 g of R6G dissolved in 10 ml of ethanol.(1mM)
• take 10 micL and 9.99ml ethanol to make 1micM R6G.

1)0.5g of 22,000 + 6 ml water

2)0.5 of 89,000-98,000 + 6 ml water

3)0.5g of 130,000 + 6 ml of water

4)0.5g of 146,000-186,000 + 6ml of water

• add 6micL of R6G in to each hydrogels. Matt Hartings there is a μL symbol you can use down below
• placed into -20C at noon.

Matt Hartings You should also add a description of your full procedure for this process here. Why are you doing these things. At some point you'll have to mention it. This (the first page of your notebook) is the best place to do it.

Notes

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