User:Samiye Yaman/Notebook/Lab 581/2013/02/01: Difference between revisions
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==Description== | ==Description== | ||
Part 1: | '''Part 1:Place the hydrogels synthesized on 30/01/2013 in to -20C''' | ||
'''Part 2: Prepare Hydrogels with 0.5mM of R6G dye''' (for detailed method, see http://openwetware.org/wiki/User:Samiye_Yaman/Notebook/Lab_581/2013/01/30 ) | |||
*0.00479 g of R6G dissolved in 10 ml of ethanol(Concentration is 1mM). Take 10 micL and 9.99ml ethanol to make 1micM R6G. | |||
1)0.5g of 22,000 + 6 ml water (A) | |||
2)0.5 of 89,000-98,000 + 6 ml water (C) | |||
3)0.5g of 130,000 + 9 ml of water (E) | |||
* | 4)0.5g of 146,000-186,000 + 9 ml of water (G) | ||
*add 6 micL of R6G in to no 1) and 2). | |||
*add 9 micL of R6G in to no 3) and 4). | |||
*transfer them into cell plates with corresponding letters (A-D) then place the 96 cell plate in to -20C at 4pm. | |||
==Data== | ==Data== |
Revision as of 09:00, 6 February 2013
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ObjectiveDescriptionPart 1:Place the hydrogels synthesized on 30/01/2013 in to -20C Part 2: Prepare Hydrogels with 0.5mM of R6G dye (for detailed method, see http://openwetware.org/wiki/User:Samiye_Yaman/Notebook/Lab_581/2013/01/30 )
1)0.5g of 22,000 + 6 ml water (A) 2)0.5 of 89,000-98,000 + 6 ml water (C) 3)0.5g of 130,000 + 9 ml of water (E) 4)0.5g of 146,000-186,000 + 9 ml of water (G)
DataNotesThis area is for any observations or conclusions that you would like to note.
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