# User:Samiye Yaman/Notebook/Lab 581/2013/02/01

(Difference between revisions)
Jump to: navigation, search
 Revision as of 13:40, 1 February 2013 (view source) (→Description)← Previous diff Current revision (12:00, 6 February 2013) (view source) (→Description) (3 intermediate revisions not shown.) Line 21: Line 21: '''Part 1:Place the hydrogels synthesized on 30/01/2013 in to -20C''' '''Part 1:Place the hydrogels synthesized on 30/01/2013 in to -20C''' - '''Part 2: Prepare Hydrogels with 0.5mM of R6G dye''' + '''Part 2: Prepare Hydrogels with 0.5mM of R6G dye''' (for detailed method,  see http://openwetware.org/wiki/User:Samiye_Yaman/Notebook/Lab_581/2013/01/30 ) - 0.00479 g of R6G dissolved in 10 ml of ethanol.(1mM) + - take 10 micL and 9.99ml ethanol to make 1micM R6G. + - 1)0.5g of 22,000 + 6 ml water + *0.00479 g of R6G dissolved in 10 ml of ethanol(Concentration is 1mM). Take 10 micL and 9.99ml ethanol to make 1micM R6G. - 2)0.5 of 89,000-98,000 + 6 ml water + 1)0.5g of 22,000 + 6 ml water (A) - 3)0.5g of 130,000 + 6 ml of water + 2)0.5 of 89,000-98,000 + 6 ml water (C) - 4)0.5g of 146,000-186,000 + 6ml of water + 3)0.5g of 130,000 + 9 ml of water (E) - *add 6micL of R6G in to each hydrogels. + 4)0.5g of 146,000-186,000 + 9 ml of water (G) - *placed into -20C at noon. + *add 6 micL of R6G in to no 1) and 2). + + *add 9 micL of R6G in to no 3) and 4). + + *transfer them into cell plates with corresponding letters (A-D) then place the 96 cell plate in to  -20C at 4pm. ==Data== ==Data==

## Current revision

Experimental Chemistry Main project page
Previous entry      Next entry

## Description

Part 1:Place the hydrogels synthesized on 30/01/2013 in to -20C

Part 2: Prepare Hydrogels with 0.5mM of R6G dye (for detailed method, see http://openwetware.org/wiki/User:Samiye_Yaman/Notebook/Lab_581/2013/01/30 )

• 0.00479 g of R6G dissolved in 10 ml of ethanol(Concentration is 1mM). Take 10 micL and 9.99ml ethanol to make 1micM R6G.

1)0.5g of 22,000 + 6 ml water (A)

2)0.5 of 89,000-98,000 + 6 ml water (C)

3)0.5g of 130,000 + 9 ml of water (E)

4)0.5g of 146,000-186,000 + 9 ml of water (G)

• add 6 micL of R6G in to no 1) and 2).
• add 9 micL of R6G in to no 3) and 4).
• transfer them into cell plates with corresponding letters (A-D) then place the 96 cell plate in to -20C at 4pm.

## Notes

This area is for any observations or conclusions that you would like to note.

Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.