User:Samiye Yaman/Notebook/Lab 581/2013/02/01

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Current revision (11:00, 6 February 2013) (view source)
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'''Part 1:Place the hydrogels synthesized on 30/01/2013 in to -20C'''
'''Part 1:Place the hydrogels synthesized on 30/01/2013 in to -20C'''
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'''Part 2: Prepare Hydrogels with 0.5mM of R6G dye'''
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'''Part 2: Prepare Hydrogels with 0.5mM of R6G dye''' (for detailed method,  see http://openwetware.org/wiki/User:Samiye_Yaman/Notebook/Lab_581/2013/01/30 )
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0.00479 g of R6G dissolved in 10 ml of ethanol.(1mM)
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take 10 micL and 9.99ml ethanol to make 1micM R6G.
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1)0.5g of 22,000 + 6 ml water
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*0.00479 g of R6G dissolved in 10 ml of ethanol(Concentration is 1mM). Take 10 micL and 9.99ml ethanol to make 1micM R6G.
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2)0.5 of 89,000-98,000 + 6 ml water
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1)0.5g of 22,000 + 6 ml water (A)
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3)0.5g of 130,000 + 9 ml of water
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2)0.5 of 89,000-98,000 + 6 ml water (C)
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4)0.5g of 146,000-186,000 + 9 ml of water
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3)0.5g of 130,000 + 9 ml of water (E)
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4)0.5g of 146,000-186,000 + 9 ml of water (G)
*add 6 micL of R6G in to no 1) and 2).
*add 6 micL of R6G in to no 1) and 2).
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*add 9 micL of R6G in to no 3) and 4).
*add 9 micL of R6G in to no 3) and 4).
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*placed into -20C at noon.
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*transfer them into cell plates with corresponding letters (A-D) then place the 96 cell plate in to  -20C at 4pm.
==Data==
==Data==

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Objective

Description

Part 1:Place the hydrogels synthesized on 30/01/2013 in to -20C

Part 2: Prepare Hydrogels with 0.5mM of R6G dye (for detailed method, see http://openwetware.org/wiki/User:Samiye_Yaman/Notebook/Lab_581/2013/01/30 )

  • 0.00479 g of R6G dissolved in 10 ml of ethanol(Concentration is 1mM). Take 10 micL and 9.99ml ethanol to make 1micM R6G.

1)0.5g of 22,000 + 6 ml water (A)

2)0.5 of 89,000-98,000 + 6 ml water (C)

3)0.5g of 130,000 + 9 ml of water (E)

4)0.5g of 146,000-186,000 + 9 ml of water (G)

  • add 6 micL of R6G in to no 1) and 2).
  • add 9 micL of R6G in to no 3) and 4).
  • transfer them into cell plates with corresponding letters (A-D) then place the 96 cell plate in to -20C at 4pm.

Data

Notes

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