User:Samiye Yaman/Notebook/Lab 581/2013/02/06: Difference between revisions
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*Add 1.25 g of sodium diphosphate and 1.34g of sodium monophosphate in to a 900ml of miliQ water. (Stir well to make sure it is completely homogeneous). Ph needs to be 7.4 so 0.5M of sodium hydroxide was added drop by drop to make sure Ph reaches to 7.4. | *Add 1.25 g of sodium diphosphate and 1.34g of sodium monophosphate in to a 900ml of miliQ water. (Stir well to make sure it is completely homogeneous). Ph needs to be 7.4 so 0.5M of sodium hydroxide was added drop by drop to make sure Ph reaches to 7.4. | ||
'''Part B Dissolving PVOH with phosphate buffer''' | |||
*0.51 g of PVOH (MW:89000-98000) was placed in to a beaker with 3 ml of water. It was placed in to a hot plate with temperature of 120C.(stir well to dissolve PVOH completely) | |||
==Data== | ==Data== | ||
==Notes== | ==Notes== |
Revision as of 08:01, 6 February 2013
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ObjectiveDescription
Part A Synthesizing Phosphate Buffer
(Base/acid)=1.25.
Mass used for base: (119.98 g/mol)* 0.01115mol= 1.338g Mass used for acid: (141.96 g/mol) * 0.00885mol= 1.256g
Part B Dissolving PVOH with phosphate buffer
DataNotes |