User:Samiye Yaman/Notebook/Lab 581/2013/02/06

(Difference between revisions)

Revision as of 11:01, 6 February 2013

Experimental Chemistry

Hydrogels synthesized on 01/02/2013 was transferred from -20C to room temperature.

Part A Synthesizing Phosphate Buffer

(Base/acid)=1.25.

Mass used for base: (119.98 g/mol)* 0.01115mol= 1.338g

Mass used for acid: (141.96 g/mol) * 0.00885mol= 1.256g

Add 1.25 g of sodium diphosphate and 1.34g of sodium monophosphate in to a 900ml of miliQ water. (Stir well to make sure it is completely homogeneous). Ph needs to be 7.4 so 0.5M of sodium hydroxide was added drop by drop to make sure Ph reaches to 7.4.

Part B Dissolving PVOH with phosphate buffer

0.51 g of PVOH (MW:89000-98000) was placed in to a beaker with 3 ml of water. It was placed in to a hot plate with temperature of 120C.(stir well to dissolve PVOH completely)

@@ Line 41: / Line 41: @@
 *Add 1.25 g of sodium diphosphate and 1.34g of sodium monophosphate in to a 900ml of miliQ water. (Stir well to make sure it is completely homogeneous). Ph needs to be 7.4 so 0.5M of sodium hydroxide was added drop by drop to make sure Ph reaches to 7.4.
+'''Part B Dissolving PVOH with phosphate buffer'''
+*0.51 g of PVOH (MW:89000-98000) was placed in to a beaker with 3 ml of water. It was placed in to a hot plate with temperature of 120C.(stir well to dissolve PVOH completely)
 ==Data==
 ==Notes==