User:Samiye Yaman/Notebook/Lab 581/2013/02/20

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(Description)
(Description)
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==Description==
==Description==
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*Prepare 3 sets of hydrogels with phosphate buffers
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*Prepare 3 sets of hydrogels with phosphate buffers
A)0.51 g of PVOH (MW:89,000-98,000) with 4 ml of phosphate buffer was placed on to a heat block at 100C.
A)0.51 g of PVOH (MW:89,000-98,000) with 4 ml of phosphate buffer was placed on to a heat block at 100C.
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*Homoginize each of the hydrogels solutions with 50 ml of mineral oil. Right after pouring in to a beaker, flash freeze it with liquid nitrogen then place them into freezer. (3 freeze thaw cycle)
*Homoginize each of the hydrogels solutions with 50 ml of mineral oil. Right after pouring in to a beaker, flash freeze it with liquid nitrogen then place them into freezer. (3 freeze thaw cycle)
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==Data==
==Data==
==Notes==
==Notes==

Revision as of 09:39, 20 February 2013

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Contents

=Objections

Description

  • Prepare 3 sets of hydrogels with phosphate buffers

A)0.51 g of PVOH (MW:89,000-98,000) with 4 ml of phosphate buffer was placed on to a heat block at 100C.

B)0.25 g of PVOH (MW:89,000-98,000) with 3 ml of phosphate buffer was placed on to a heat block at 100C.

C)0.14 g of PVOH (MW:89,000-98,000) with 3 ml of phosphate buffer was placed on to a heat block at 100C.

  • Add 2μ of R6G were added for each ml of phophate buffer. Add 6μ R6G in to B and C. Add 8μ to A.
  • Homoginize each of the hydrogels solutions with 50 ml of mineral oil. Right after pouring in to a beaker, flash freeze it with liquid nitrogen then place them into freezer. (3 freeze thaw cycle)

Data

Notes

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