User:Samiye Yaman/Notebook/Lab 581/2013/02/20
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==Description== | ==Description== | ||
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| + | '''Part 1''' | ||
*Prepare 3 sets of hydrogels with phosphate buffers | *Prepare 3 sets of hydrogels with phosphate buffers | ||
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*Add 2μ of R6G were added for each ml of phophate buffer. Add 6μ R6G in to B and C. Add 8μ to A. | *Add 2μ of R6G were added for each ml of phophate buffer. Add 6μ R6G in to B and C. Add 8μ to A. | ||
| - | *Homoginize each of the hydrogels solutions with | + | *Homoginize each of the hydrogels solutions with 35 ml of safflower oil. Right after pouring in to a beaker, flash freeze it with liquid nitrogen then place them into freezer. (3 freeze thaw cycle) |
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| + | '''Part 2''' | ||
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| + | *Prepare 3 sets of hydrogels with phosphate buffers | ||
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| + | A)0.52 g of PVOH (MW:89,000-98,000) with 4 ml of phosphate buffer was placed on to a heat block at 100C. | ||
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| + | B)0.25 g of PVOH (MW:89,000-98,000) with 3 ml of phosphate buffer was placed on to a heat block at 100C. | ||
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| + | C)0.13 g of PVOH (MW:89,000-98,000) with 3 ml of phosphate buffer was placed on to a heat block at 100C. | ||
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| + | *Homoginize each of the hydrogels solutions with 50 ml of safflower oil. Pour them in to a petri dish to have a larger surface area then place them into freezer.(-20C) (3 freeze thaw cycle) | ||
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| + | '''Part 3''' | ||
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| + | *Prepare a set of hydrogel with phosphate buffer | ||
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| + | A)0.25 g POVH (MW:89,000-98,000) with 4 ml of phosphate buffer was blaced to a heat block at 100C. | ||
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| + | *Homoginize each of the hydrogels solutions with 50 ml of mineral oil. Pour them in to a petri dish to have a larger surface area then place it into the freezer.(-20C) (3 freeze thaw cycle) | ||
==Data== | ==Data== | ||
==Notes== | ==Notes== | ||
Revision as of 12:21, 20 February 2013
 Experimental Chemistry
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ObjectionsDescriptionPart 1
A)0.51 g of PVOH (MW:89,000-98,000) with 4 ml of phosphate buffer was placed on to a heat block at 100C. B)0.25 g of PVOH (MW:89,000-98,000) with 3 ml of phosphate buffer was placed on to a heat block at 100C. C)0.14 g of PVOH (MW:89,000-98,000) with 3 ml of phosphate buffer was placed on to a heat block at 100C.
A)0.52 g of PVOH (MW:89,000-98,000) with 4 ml of phosphate buffer was placed on to a heat block at 100C. B)0.25 g of PVOH (MW:89,000-98,000) with 3 ml of phosphate buffer was placed on to a heat block at 100C. C)0.13 g of PVOH (MW:89,000-98,000) with 3 ml of phosphate buffer was placed on to a heat block at 100C.
A)0.25 g POVH (MW:89,000-98,000) with 4 ml of phosphate buffer was blaced to a heat block at 100C.
DataNotes |
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