User:Samiye Yaman/Notebook/Lab 581/2013/03/01

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(Description)
(Description)
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*Add 6ml of water and sonicate the samples.
*Add 6ml of water and sonicate the samples.
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*Take 1 ml of each sample, centrifuge it. Additionally pipette it up and down to break the larger pieces.
+
*Take 1 ml of each sample add in to 1.5ml microcentrifuge tubes. Centrifuge samples.( Additionally pipette it up and down to break the larger pieces.)
-
*Add 1μl of R6G to each epi test tubes to be absorbed by the PVOH.
+
*Add 1μl of R6G to each microcentrifuge tubes to be absorbed by the PVOH.
*Fluorescence of samples with dyes already attached were measured by using fluorometer.
*Fluorescence of samples with dyes already attached were measured by using fluorometer.

Revision as of 15:22, 1 March 2013

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Contents

Objections

Description

Part 1

  • Place a small piece of the hydrogels (synthesized on 22/02/2012) in a water solution.
  • Observe under the microscope.
  • Sonicate each samples.
  • Fluorescence of samples with dyes already attached were measured by using fluorometer.

Part 2

  • 0.3 g of sample with and without lecithin placed in to 2 different vials.
  • Add 6ml of water and sonicate the samples.
  • Take 1 ml of each sample add in to 1.5ml microcentrifuge tubes. Centrifuge samples.( Additionally pipette it up and down to break the larger pieces.)
  • Add 1μl of R6G to each microcentrifuge tubes to be absorbed by the PVOH.
  • Fluorescence of samples with dyes already attached were measured by using fluorometer.

Data

Notes

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