User:Samiye Yaman/Notebook/Lab 581/2013/03/20: Difference between revisions

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==Objectives==
==Objectives==


'''Part A'''  
'''Part A: Prepare tris-HCl Buffer'''
 
*25mmol/L x 1mol/1000mmmol x 1L/1000mL x 300mL x 121.14g/mol
 
= 0.90855g tris
 
*Add 250ml of distilled water to the beaker with 0.9094g of tris.
 
*Add HCl drop by drop until the pH was at 8.07.
 
*47ml of more distilled water was added to have 300ml of 25mM tris-HCl buffer.
 
'''Part B: Preparing hydrogels for liposome coating'''
 
*Add 0.2mL lipid (4mg) with 2 mg hydrogels.(1. 0.5g PVOH/3mL buffer/6μL R6G/50mL oil 2. 0.5g PVOH/4mL buffer/35mL oil)
 
*Gently stir for 2 hours.


==Description==
==Description==

Revision as of 08:34, 27 March 2013

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Objectives

Part A: Prepare tris-HCl Buffer

  • 25mmol/L x 1mol/1000mmmol x 1L/1000mL x 300mL x 121.14g/mol

= 0.90855g tris

  • Add 250ml of distilled water to the beaker with 0.9094g of tris.
  • Add HCl drop by drop until the pH was at 8.07.
  • 47ml of more distilled water was added to have 300ml of 25mM tris-HCl buffer.

Part B: Preparing hydrogels for liposome coating

  • Add 0.2mL lipid (4mg) with 2 mg hydrogels.(1. 0.5g PVOH/3mL buffer/6μL R6G/50mL oil 2. 0.5g PVOH/4mL buffer/35mL oil)
  • Gently stir for 2 hours.

Description

Data

Notes
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