User:Samiye Yaman/Notebook/Lab 581/2013/03/22: Difference between revisions

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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Experimental Chemistry</span>
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Experimental Chemistry</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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==Description==
==Description==


'''Part A'''
'''Preparing hydrogels for pH analysis'''
 
*Add 1.5ml of water in to a epitest tube with 2mg hydrogel.
 
*Centrifuge sample containing hydrogels and crystal violet indicator 3 times for 5 minutes at 13200 rpm.
 
*By using UV-Vis, measure the absorbance of each supernatant to find the amount of crytal violet in the supernatent. Use the data to find out the amount of crystal violet absorbed by the hydrogels.(Initial amount of indicator added to the epi test tube is 20uL)
 
*Prepare 2 more hydrogel solutions (with 1.5 water and 2mg hydrogel) with 55 μL methyl red and 30μL bromophenol blue.
 
'''Info on indicators'''
 
methyl violet 0-2
 
methyl red- 4.4-6.2
 
bromocresol purple- 5.2-6.8
 
bromophenol blue 6.0-7.6
 
cresol red 7.2-8.8
 
phenolphthalein- 8.3-10.0
 
==Data==
==Data==
[[Image:Crystal_violet_leakage.png]]


==Notes==
==Notes==

Latest revision as of 22:34, 26 September 2017

Experimental Chemistry Main project page
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Objective

Description

Preparing hydrogels for pH analysis

  • Add 1.5ml of water in to a epitest tube with 2mg hydrogel.
  • Centrifuge sample containing hydrogels and crystal violet indicator 3 times for 5 minutes at 13200 rpm.
  • By using UV-Vis, measure the absorbance of each supernatant to find the amount of crytal violet in the supernatent. Use the data to find out the amount of crystal violet absorbed by the hydrogels.(Initial amount of indicator added to the epi test tube is 20uL)
  • Prepare 2 more hydrogel solutions (with 1.5 water and 2mg hydrogel) with 55 μL methyl red and 30μL bromophenol blue.

Info on indicators

methyl violet 0-2

methyl red- 4.4-6.2

bromocresol purple- 5.2-6.8

bromophenol blue 6.0-7.6

cresol red 7.2-8.8

phenolphthalein- 8.3-10.0

Data

Notes
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