User:Samiye Yaman/Notebook/Lab 581/2013/03/29: Difference between revisions
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Experimental Chemistry</span> | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Experimental Chemistry</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Objectives== | |||
==Description== | ==Description== | ||
* | '''Part A: Preparing Dilutions for UV-Vis Measurements''' | ||
*Centrifuge 6 solutions containing varying amounts of R6G prepared on 03/27/2013 for 5 minutes at 11200rpm 3 times. | |||
*Prepare stock solutions for comprison (10μL, 15μL, 20μL, 25μL, 30μL and 35μL in 1.5 ml phosphate buffer) | |||
* UV-Vis measurements of stock solutions and supernatants were taken | |||
'''Part B: Observing pH changes''' | |||
*Separate crystal violet containing hydrogels into two epi-test tubes, labelled as A & B.(app 1mg in each) | |||
*Add 1mL of 1M HCl to one epi test tube A and 1mL NaOH to epitest tube B. | |||
3) Centrifuge for 2 min at 11200rpm. | |||
4) Take UV-Vis of supernatants | |||
'''Part C: Prepare hydrogels with further pH analysis''' | |||
*0.5mg samples of PVOH hydrogels containing no lecithin and no dye was added in to 3 seperate epi test tubes with 1.5 mL phosphate buffer. | |||
==Data== | |||
[[Image:Graf_10ul.png]] | |||
[[Image:15ul.png]] | |||
[[Image:20ul.png]] | |||
[[Image:Graf_25ul.png]] | |||
[[Image:30ul.png]] | |||
[[Image:35ul.png]] | |||
==Notes== |
Latest revision as of 22:35, 26 September 2017
Experimental Chemistry | Main project page Previous entry Next entry |
ObjectivesDescriptionPart A: Preparing Dilutions for UV-Vis Measurements
Part B: Observing pH changes
3) Centrifuge for 2 min at 11200rpm. 4) Take UV-Vis of supernatants Part C: Prepare hydrogels with further pH analysis
DataNotes |