User:Samiye Yaman/Notebook/Lab 581/2013/03/29: Difference between revisions
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==Description== | ==Description== | ||
'''Part A: Preparing Dilutions for UV-Vis Measurements''' | |||
*Centrifuge 6 solutions containing varying amounts of R6G prepared on 03/27/2013 for 5 minutes at 11200rpm 3 times. | |||
*Prepare stock solutions for comprison (10μL, 15μL, 20μL, 25μL, 30μL and 35μL in 1.5 ml phosphate buffer) | |||
* UV-Vis measurements of stock solutions and supernatants were taken | |||
'''Part B: Observing pH changes''' | |||
*Separate crystal violet containing hydrogels into two epi-test tubes, labelled as A & B.(app 1mg in each) | |||
*Add 1mL of 1M HCl to one epi test tube A and 1mL NaOH to epitest tube B. | |||
3) Centrifuge for 2 min at 11200rpm. | |||
4) Take UV-Vis of supernatants | |||
'''Part C: Prepare hydrogels with further pH analysis''' | |||
*0.5mg samples of PVOH hydrogels containing no lecithin and no dye was added in to 3 seperate epi test tubes with 1.5 mL phosphate buffer. | |||
==Data== | ==Data== | ||
==Notes== | ==Notes== | ||
Revision as of 14:24, 6 April 2013
 Experimental Chemistry
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ObjectivesDescriptionPart A: Preparing Dilutions for UV-Vis Measurements
Part B: Observing pH changes
3) Centrifuge for 2 min at 11200rpm. 4) Take UV-Vis of supernatants Part C: Prepare hydrogels with further pH analysis
DataNotes |
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