User:Sanuja Mohanaraj/Notebook/Experimental Biochemistry 1 Fall 2017/2017/08/30: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Amino Acid Stock Solutions Preparation== | ==Amino Acid Stock Solutions Preparation== | ||
First, three amino acids are selected before preparing each stock solution: L-Isoleucine (aliphatic), Arginine (amine) and Phenylalanine (aromatic). Next, 1.6398mg of L-isoleucine is measured out into a weighing boat and transferred into a 50mL volumetric flask. Deionized water is then added to the volumetric flask to the mark, achieving a final concentration of 250μM. The volumetric flask in then sealed using parafilm, and stored. | First, three amino acids are selected before preparing each stock solution: L-Isoleucine (aliphatic), Arginine (amine) and Phenylalanine (aromatic). Next, 1.6398mg of L-isoleucine is measured out into a weighing boat and transferred into a 50mL volumetric flask. Deionized water is then added to the volumetric flask to the mark, achieving a final concentration of 250μM. The volumetric flask in then sealed using parafilm, and stored. | ||
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This process is repeated using separate 50mL volumetric flasks, each time using 2.1775mg of Arginine and 2.0649mg of Phenylalanine. Finally, once all three solutions are prepared, the absorbance and fluorescence of each amino acid stock solution are measured. | This process is repeated using separate 50mL volumetric flasks, each time using 2.1775mg of Arginine and 2.0649mg of Phenylalanine. Finally, once all three solutions are prepared, the absorbance and fluorescence of each amino acid stock solution are measured. | ||
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Three different molar ratios of the gold nanoparticles and each amino acid solution (Au:AA) are prepared, and the pH of each solution is then measured as follows: | Three different molar ratios of the gold nanoparticles and each amino acid solution (Au:AA) are prepared, and the pH of each solution is then measured as follows: | ||
'''Phenylalanine (aromatic)''' | '''Phenylalanine (aromatic)'''<br> | ||
Ratio 1:20 -- pH 3.327<br> | |||
Ratio 1:20 -- pH 3.327 | Ratio 1:80 -- pH 3.273<br> | ||
Ratio 1:80 -- pH 3.273 | |||
Ratio 1:160 -- pH 3.244 | Ratio 1:160 -- pH 3.244 | ||
'''L-isoleucine (aliphatic)''' | '''L-isoleucine (aliphatic)'''<br> | ||
Ratio 1:20 -- pH 3.235<br> | |||
Ratio 1:20 -- pH 3.235 | Ratio 1:80 -- pH 3.292<br> | ||
Ratio 1:80 -- pH 3.292 | |||
Ratio 1:160 -- pH 3.338 | Ratio 1:160 -- pH 3.338 | ||
'''Arginine (amine)''' | '''Arginine (amine)'''<br> | ||
Ratio 1:20 -- pH 3.372<br> | |||
Ratio 1:20 -- pH 3.372 | Ratio 1:80 -- pH 3.260<br> | ||
Ratio 1:80 -- pH 3.260 | |||
Ratio 1:160 -- pH 3.272 | Ratio 1:160 -- pH 3.272 | ||
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== | == Results== | ||
The solutions that were made did not form gold nanoparticles, so the solutions were remade with a lower Au:AA molar ratios over a different pH range, the following week. | |||
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__NOTOC__ | __NOTOC__ |
Latest revision as of 16:25, 20 November 2017
Project name | Main project page Previous entry Next entry |
Amino Acid Stock Solutions PreparationFirst, three amino acids are selected before preparing each stock solution: L-Isoleucine (aliphatic), Arginine (amine) and Phenylalanine (aromatic). Next, 1.6398mg of L-isoleucine is measured out into a weighing boat and transferred into a 50mL volumetric flask. Deionized water is then added to the volumetric flask to the mark, achieving a final concentration of 250μM. The volumetric flask in then sealed using parafilm, and stored.
Au Nanoparticles Solutions PreparationThree different molar ratios of the gold nanoparticles and each amino acid solution (Au:AA) are prepared, and the pH of each solution is then measured as follows: Phenylalanine (aromatic) L-isoleucine (aliphatic) Arginine (amine) Calculationsmoles / volume = molarity L-isoleucine Phenylalanine Arginine
ResultsThe solutions that were made did not form gold nanoparticles, so the solutions were remade with a lower Au:AA molar ratios over a different pH range, the following week.
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