User:Sanuja Mohanaraj/Notebook/Experimental Biochemistry 1 Fall 2017/2017/08/30
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Amino Acid Stock Solutions PreparationFirst, three amino acids are selected before preparing each stock solution: L-Isoleucine (aliphatic), Arginine (amine) and Phenylalanine (aromatic). Next, 1.6398mg of L-isoleucine is measured out into a weighing boat and transferred into a 50mL volumetric flask. Deionized water is then added to the volumetric flask to the mark, achieving a final concentration of 250μM. The volumetric flask in then sealed using parafilm, and stored. This process is repeated using separate 50mL volumetric flasks, each time using 2.1775mg of Arginine and 2.0649mg of Phenylalanine. Finally, once all three solutions are prepared, the absorbance and fluorescence of each amino acid stock solution are measured. Au Nanoparticles Solutions PreparationThree different molar ratios of the gold nanoparticles and each amino acid solution (Au:AA) are prepared, and the pH of each solution is then measured as follows: Phenylalanine (aromatic) Ratio 1:20 -- pH 3.327 Ratio 1:80 -- pH 3.273 Ratio 1:160 -- pH 3.244 L-isoleucine (aliphatic) Ratio 1:20 -- pH 3.235 Ratio 1:80 -- pH 3.292 Ratio 1:160 -- pH 3.338 Arginine (amine) Ratio 1:20 -- pH 3.372 Ratio 1:80 -- pH 3.260 Ratio 1:160 -- pH 3.272 Calculationsmoles / volume = molarity L-isoleucine Phenylalanine Arginine
UV-Absorbace & Fluorescence Spectra |
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