User:Sara K. Mattson/Notebook/Chemistry 671/2016/10/26: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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#The scan range parameters were start at 310nm and ending at 540nm | #The scan range parameters were start at 310nm and ending at 540nm | ||
#The emission and exit slit were set at 10nm | #The emission and exit slit were set at 10nm | ||
==Data== | |||
Scans AuNP pH 8 | |||
[[Image:Myoglobin pH8 fluorescence.png|600px]] | |||
Wavelength of Myoglobin AuNp's at pH 8 | |||
[[Image:Myoglobin pH8 wavelength.png|600px]] | |||
Maximum Intensity of Myoglobin AuNp's at pH 8 | |||
[[Image:Myoglobin pH8 integrated intensity.png|600px]] | |||
Integrated Intensity of Myoglobins AuNp's at pH 8 | |||
[[Image:Myoglobin pH8 maximum intensity.png|600px]] | |||
==Solution for UV-Vis Preparation== | ==Solution for UV-Vis Preparation== | ||
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Stock Concentrations: | Stock Concentrations: | ||
#Myoglobin | #Myoglobin 70uM for 0.75mM Fructose solutions, 67.76uM for 1.25mM Fructose solution | ||
#Silver 25.8mM | #Silver 25.8mM | ||
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{|border="1" cellpadding="2" | {|border="1" cellpadding="2" | ||
|+ 0. | |+ 0.75mM Fructose | ||
! scope="col" width="50" | pH | ! scope="col" width="50" | pH | ||
! scope="col" width="100" | μL of 1 mM HNO3<sub>stock</sub> | ! scope="col" width="100" | μL of 1 mM HNO3<sub>stock</sub> | ||
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! scope="col" width="100" | μL of Water | ! scope="col" width="100" | μL of Water | ||
|- | |- | ||
| 4 || 500 || 0 || 0 || 48 || | | 4 || 500 || 0 || 0 || 48 || 113 || 886 || 3451 | ||
|- | |- | ||
| 5 || 50 || 0 || 0 || 48 || | | 5 || 50 || 0 || 0 || 48 || 113 || 886 || 3901 | ||
|- | |- | ||
| 6 || 5 || 0 || 0 || 48 || | | 6 || 5 || 0 || 0 || 48 || 113 || 886 || 3946 | ||
|- | |- | ||
| 7 || 0 || 0 || 0 || 48 || | | 7 || 0 || 0 || 0 || 48 || 113 || 886 || 3951 | ||
|- | |- | ||
| 8 || 0 || 5 || 0 || 48 || | | 8 || 0 || 5 || 0 || 48 || 113 || 886 || 3946 | ||
|- | |- | ||
| 9 || 0 || 50 || 0 || 48 || | | 9 || 0 || 50 || 0 || 48 || 113 || 886 || 3901 | ||
|- | |- | ||
| 10 || 0 || 500 || 0 || 48 || | | 10 || 0 || 500 || 0 || 48 || 113 || 886 || 3451 | ||
|- | |- | ||
| 11 || 0 || 0 || 5 || 48 || | | 11 || 0 || 0 || 5 || 48 || 113 || 886 || 3946 | ||
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| 12 || 0 || 0 || 50 || 48 || | | 12 || 0 || 0 || 50 || 48 || 113 || 886 || 3901 | ||
|} | |} | ||
{|border="1" cellpadding="2" | {|border="1" cellpadding="2" | ||
|+ | |+ 1.25 mM Fructose | ||
! scope="col" width="50" | pH | ! scope="col" width="50" | pH | ||
! scope="col" width="100" | μL of 1 mM HNO3<sub>stock</sub> | ! scope="col" width="100" | μL of 1 mM HNO3<sub>stock</sub> | ||
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! scope="col" width="100" | μL of Water | ! scope="col" width="100" | μL of Water | ||
|- | |- | ||
| 4 || 500 || 0 || 0 || 48 || | | 4 || 500 || 0 || 0 || 48 || 189 || 922 || 3339 | ||
|- | |- | ||
| 5 || 50 || 0 || 0 || 48 || | | 5 || 50 || 0 || 0 || 48 || 189 || 922 || 3789 | ||
|- | |- | ||
| 6 || 5 || 0 || 0 || 48 || | | 6 || 5 || 0 || 0 || 48 || 189 || 922 || 3834 | ||
|- | |- | ||
| 7 || 0 || 0 || 0 || 48 || | | 7 || 0 || 0 || 0 || 48 || 189 || 922 || 3839 | ||
|- | |- | ||
| 8 || 0 || 5 || 0 || 48 || | | 8 || 0 || 5 || 0 || 48 || 189 || 922 || 3834 | ||
|- | |- | ||
| 9 || 0 || 50 || 0 || 48 || | | 9 || 0 || 50 || 0 || 48 || 189 || 922 || 3787 | ||
|- | |- | ||
| 10 || 0 || 500 || 0 || 48 || | | 10 || 0 || 500 || 0 || 48 || 189 || 922 || 3339 | ||
|- | |- | ||
| 11 || 0 || 0 || 5 || 48 || | | 11 || 0 || 0 || 5 || 48 || 189 || 922 || 3834 | ||
|- | |- | ||
| 12 || 0 || 0 || 50 || 48 || | | 12 || 0 || 0 || 50 || 48 || 189 || 922 || 3789 | ||
|} | |} | ||
Latest revision as of 02:07, 27 September 2017
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FluorescencePurposeUse fluorescence to examine the rate at which Myoglobin templated gold nanoparticles unfold at pH 8 Procedure
DataScans AuNP pH 8 Wavelength of Myoglobin AuNp's at pH 8 Maximum Intensity of Myoglobin AuNp's at pH 8 Integrated Intensity of Myoglobins AuNp's at pH 8 Solution for UV-Vis PreparationStock Concentrations:
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