User:Sara K. Mattson/Notebook/Chemistry 671/2016/11/30: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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##Water
##Water
###Volume of water= 3000uL- 223uL-163uL-30uL= 2584uL
###Volume of water= 3000uL- 223uL-163uL-30uL= 2584uL


==Data==
==Data==

Latest revision as of 02:10, 27 September 2017

Project name Main project page
Previous entry      Next entry

Ocean Optics

Purpose

To use ocean optics to make conclusions about the rate of myoglobin templated Au nanoparticle formation in pH 11 and 12

Procedure

First pH 11 was made:

  1. Sample was made Myoglobin: 0.25mM Au, 12.5uM Myoglobin, 3mL total
    1. Au 3.37mM
      1. (0.25mM)(3.0mL)=(XmL)(3.37mM)
      2. XmL= .223mL= 223uL Au
    2. Myoglobin 230uM
      1. (12.5uM)(3.0mL)=(XmL)(230uM)
      2. X=0.163mL= 163uL Myoglobin
    3. 3uL 1M NaOH
    4. Water
      1. Volume of water= 3000uL- 223uL-163uL-3uL= 2611uL
    5. Scans were taken at 80oC every 2 minutes for 3 hours

Second pH 12 was made:

  1. Sample was made Myoglobin: 0.25mM Au, 12.5uM Myoglobin, 3mL total
    1. Au 3.37mM
      1. (0.25mM)(3.0mL)=(XmL)(3.37mM)
      2. XmL= .223mL= 223uL Au
    2. Myoglobin 230uM
      1. (12.5uM)(3.0mL)=(XmL)(230uM)
      2. X=0.163mL= 163uL Myoglobin
    3. 30uL 1M NaOH
    4. Water
      1. Volume of water= 3000uL- 223uL-163uL-30uL= 2584uL

Data

pH 11 Data

Absorbance

Wavelength


pH 12 Data

Absorbance

Wavelength


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