User:Sara K. Mattson/Notebook/Chemistry 671/2016/12/06: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==Fluorescence== | ||
==Purpose== | |||
Use fluorescence to examine the rate at which Myoglobin templated gold nanoparticles unfold at pH 11 and pH 12 | |||
==Procedure== | |||
pH 11 | |||
#Sample was made Myoglobin: 0.25mM Au, 12.5uM Myoglobin, 3mL total | |||
##Au 3.37mM | |||
###(0.25mM)(3.0mL)=(XmL)(3.37mM) | |||
###XmL= .223mL= 223uL Au | |||
##Myoglobin 230uM | |||
###(12.5uM)(3.0mL)=(XmL)(230uM) | |||
###X=0.163mL= 163uL Myoglobin | |||
##3uL 1mM NaOH | |||
##Water | |||
###Volume of water= 3000uL- 223uL-163uL-3uL= 2611uL | |||
#Absorbance measurements were taken and saved every 3 minutes for 3 hours | |||
#The instrument was set at 80 degrees Celsius | |||
#The scan range parameters were start at 310nm and ending at 540nm | |||
#The emission and exit slit were set at 10nm | |||
pH 12 | |||
#Sample was made Myoglobin: 0.25mM Au, 12.5uM Myoglobin, 3mL total | |||
##Au 3.37mM | |||
###(0.25mM)(3.0mL)=(XmL)(3.37mM) | |||
###XmL= .223mL= 223uL Au | |||
##Myoglobin 230uM | |||
###(12.5uM)(3.0mL)=(XmL)(230uM) | |||
###X=0.163mL= 163uL Myoglobin | |||
##30uL 1mM NaOH | |||
##Water | |||
###Volume of water= 3000uL- 223uL-163uL-30uL= 2584uL | |||
#Absorbance measurements were taken and saved every 3 minutes for 3 hours | |||
#The instrument was set at 80 degrees Celsius | |||
#The scan range parameters were start at 310nm and ending at 540nm | |||
#The emission and exit slit were set at 10nm | |||
==Data== | |||
Data for pH 11 | |||
pH 11 Scans | |||
[[Image:PH11 fluorescence scans.png|600px]] | |||
pH 11 Wavelength | |||
[[Image:PH 11 fluorescence wavelength.png|600px]] | |||
pH 11 Integrated Intensity | |||
[[Image:PH 11 fluorescence integrated intensity.png|600px]] | |||
Data for pH 12 | |||
pH 12 Scans | |||
[[Image:Fluorescence scans for pH 12.png|600px]] | |||
pH 12 Wavelength | |||
[[Image:PH 12 wavelength fluorescence.png|600px]] | |||
pH 12 Integrated Intensity | |||
[[Image:PH 12 integrated intensity.png|600px]] | |||
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Latest revision as of 02:11, 27 September 2017
Project name | Main project page Previous entry |
FluorescencePurposeUse fluorescence to examine the rate at which Myoglobin templated gold nanoparticles unfold at pH 11 and pH 12 ProcedurepH 11
pH 12
DataData for pH 11 pH 11 Scans pH 11 Wavelength pH 11 Integrated Intensity Data for pH 12
pH 12 Wavelength pH 12 Integrated Intensity |