User:Sara K. Mattson/Notebook/Chemistry 671/2016/12/06: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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#The scan range parameters were start at 310nm and ending at 540nm  
#The scan range parameters were start at 310nm and ending at 540nm  
#The emission and exit slit were set at 10nm
#The emission and exit slit were set at 10nm


==Data==
==Data==


Data for pH 11
Data for pH 11
pH 11 Scans
[[Image:PH11 fluorescence scans.png|600px]]
pH 11 Wavelength
[[Image:PH 11 fluorescence wavelength.png|600px]]
pH 11 Integrated Intensity
[[Image:PH 11 fluorescence integrated intensity.png|600px]]
Data for pH 12
pH 12 Scans
[[Image:Fluorescence scans for pH 12.png|600px]]
pH 12 Wavelength
[[Image:PH 12 wavelength fluorescence.png|600px]]
pH 12 Integrated Intensity
[[Image:PH 12 integrated intensity.png|600px]]


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Latest revision as of 02:11, 27 September 2017

Project name Main project page
Previous entry      

Fluorescence

Purpose

Use fluorescence to examine the rate at which Myoglobin templated gold nanoparticles unfold at pH 11 and pH 12

Procedure

pH 11

  1. Sample was made Myoglobin: 0.25mM Au, 12.5uM Myoglobin, 3mL total
    1. Au 3.37mM
      1. (0.25mM)(3.0mL)=(XmL)(3.37mM)
      2. XmL= .223mL= 223uL Au
    2. Myoglobin 230uM
      1. (12.5uM)(3.0mL)=(XmL)(230uM)
      2. X=0.163mL= 163uL Myoglobin
    3. 3uL 1mM NaOH
    4. Water
      1. Volume of water= 3000uL- 223uL-163uL-3uL= 2611uL
  2. Absorbance measurements were taken and saved every 3 minutes for 3 hours
  3. The instrument was set at 80 degrees Celsius
  4. The scan range parameters were start at 310nm and ending at 540nm
  5. The emission and exit slit were set at 10nm

pH 12

  1. Sample was made Myoglobin: 0.25mM Au, 12.5uM Myoglobin, 3mL total
    1. Au 3.37mM
      1. (0.25mM)(3.0mL)=(XmL)(3.37mM)
      2. XmL= .223mL= 223uL Au
    2. Myoglobin 230uM
      1. (12.5uM)(3.0mL)=(XmL)(230uM)
      2. X=0.163mL= 163uL Myoglobin
    3. 30uL 1mM NaOH
    4. Water
      1. Volume of water= 3000uL- 223uL-163uL-30uL= 2584uL
  2. Absorbance measurements were taken and saved every 3 minutes for 3 hours
  3. The instrument was set at 80 degrees Celsius
  4. The scan range parameters were start at 310nm and ending at 540nm
  5. The emission and exit slit were set at 10nm

Data

Data for pH 11

pH 11 Scans

pH 11 Wavelength

pH 11 Integrated Intensity

Data for pH 12


pH 12 Scans

pH 12 Wavelength

pH 12 Integrated Intensity


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