User:Sara K. Mattson/Notebook/Chemistry 671/2016/12/06: Difference between revisions

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pH 11 Wavelength  
pH 11 Wavelength  
[[Image:PH 11 fluorescence wavelength.png|600px]]
[[Image:PH 11 fluorescence wavelength.png|600px]]
pH 11 Integrated Intensity


[[Image:PH 11 fluorescence integrated intensity.png|600px]]
[[Image:PH 11 fluorescence integrated intensity.png|600px]]

Revision as of 11:45, 6 December 2016

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Fluorescence

Purpose

Use fluorescence to examine the rate at which Myoglobin templated gold nanoparticles unfold at pH 11 and pH 12

Procedure

pH 11

  1. Sample was made Myoglobin: 0.25mM Au, 12.5uM Myoglobin, 3mL total
    1. Au 3.37mM
      1. (0.25mM)(3.0mL)=(XmL)(3.37mM)
      2. XmL= .223mL= 223uL Au
    2. Myoglobin 230uM
      1. (12.5uM)(3.0mL)=(XmL)(230uM)
      2. X=0.163mL= 163uL Myoglobin
    3. 3uL 1mM NaOH
    4. Water
      1. Volume of water= 3000uL- 223uL-163uL-3uL= 2611uL
  2. Absorbance measurements were taken and saved every 3 minutes for 3 hours
  3. The instrument was set at 80 degrees Celsius
  4. The scan range parameters were start at 310nm and ending at 540nm
  5. The emission and exit slit were set at 10nm

pH 12

  1. Sample was made Myoglobin: 0.25mM Au, 12.5uM Myoglobin, 3mL total
    1. Au 3.37mM
      1. (0.25mM)(3.0mL)=(XmL)(3.37mM)
      2. XmL= .223mL= 223uL Au
    2. Myoglobin 230uM
      1. (12.5uM)(3.0mL)=(XmL)(230uM)
      2. X=0.163mL= 163uL Myoglobin
    3. 30uL 1mM NaOH
    4. Water
      1. Volume of water= 3000uL- 223uL-163uL-30uL= 2584uL
  2. Absorbance measurements were taken and saved every 3 minutes for 3 hours
  3. The instrument was set at 80 degrees Celsius
  4. The scan range parameters were start at 310nm and ending at 540nm
  5. The emission and exit slit were set at 10nm


Data

Data for pH 11

pH 11 Scans

pH 11 Wavelength

pH 11 Integrated Intensity


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