User:Sarah Burkhard/Notebook/471 Nano Notebook/2016/09/07: Difference between revisions

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### Appropriate amount of fructose (0.125 mM, 0.25 mM)
### Appropriate amount of fructose (0.125 mM, 0.25 mM)
### water
### water
*'''[[User:Matt Hartings|Matt Hartings]] 14:02, 9 September 2016 (EDT)''': I remade your samples because you got strange results. Here's my [[User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2016/09/09|description]]


==Data==
==Data==

Revision as of 11:02, 9 September 2016

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Fluorescence

Objective

Draw conclusions on reaction speed of nanoparticle formation from absorption spectra taking in small time intervals of 5 minutes over a period of 3 hours.

protein: BSA | pH: 4

Protocol

Prepare solutions. Gold, protein, acid , water .

  1. BSA Fluorescence
    1. 3 mL total
      1. 0.25 mM Au
      2. 3.125 μM BSA
      3. HCl or NaOH appropriate for your pH (pH = 4)
      4. water

M1V1 = M2V2 was used for calculations (Anneliese's spreadsheet)

A table of used concentration and volumes can be found here.

Preparation of stock solutions for UV-Vis:

    1. 5 mL total
      1. 0.25 mM Au
      2. 3.125 μM BSA
      3. HCl or NaOH appropriate for your pH (pH 4 - 12)
      4. Appropriate amount of fructose (0.125 mM, 0.25 mM)
      5. water
  • Matt Hartings 14:02, 9 September 2016 (EDT): I remade your samples because you got strange results. Here's my description

Data

Data Analysis

The absorbance peak will shift to higher wavelengths, due to protein unfolding. Gold particles are fluorescent, but differently as long as they are folded in the Protein (related to the low dielectric constant within the protein.) The absorbance shift occurred at ...... The absorbance was maximum at time and wavelength .....

Graphs:


Notes

BSA stock solutions go 4 hours in oven, up to 80 C. menu > hill icon > menu > choose program 1 > 1 loop


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