User:Sarah Burkhard/Notebook/471 Nano Notebook/2016/09/21: Difference between revisions
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==Solutions== | ==Solutions== | ||
{|border="1" cellpadding="2" | |||
|+ 0.75 mM Fructose | |||
! scope="col" width="50" | pH | |||
! scope="col" width="100" | μL of 1 mM HCl<sub>stock</sub> | |||
! scope="col" width="100" | μL of 1 mM NaOH<sub>stock</sub> | |||
! scope="col" width="100" | μL of 1 M NaOH<sub>stock</sub> | |||
! scope="col" width="100" | μL of AuCl<sub>3stock</sub> | |||
! scope="col" width="100" | μL of Fructose<sub>stock</sub> | |||
! scope="col" width="100" | μL of BSA<sub>stock</sub> | |||
! scope="col" width="100" | μL of Water | |||
|- | |||
| 4 || 500 || 0 || 0 || 335 || 253 || 316 || 3596 | |||
|- | |||
| 5 || 50 || 0 || 0 || 335 || 253 || 316 || 4046 | |||
|- | |||
| 6 || 5 || 0 || 0 || 335 || 253 || 316 || 4091 | |||
|- | |||
| 7 || 0 || 0 || 0 || 335 || 253 || 316 || 4096 | |||
|- | |||
| 8 || 0 || 5 || 0 || 335 || 253 || 316 || 4091 | |||
|- | |||
| 9 || 0 || 50 || 0 || 335 || 253 || 316 || 4046 | |||
|- | |||
| 10 || 0 || 500 || 0 || 335 || 253 || 316 || 3596 | |||
|- | |||
| 11 || 0 || 0 || 5 || 335 || 253 || 316 || 4091 | |||
|- | |||
| 12 || 0 || 0 || 50 || 335 || 253 || 316 || 4046 | |||
|} | |||
{|border="1" cellpadding="2" | |||
|+ 1.25 mM Fructose | |||
! scope="col" width="50" | pH | |||
! scope="col" width="100" | μL of 1 mM HCl<sub>stock</sub> | |||
! scope="col" width="100" | μL of 1 mM NaOH<sub>stock</sub> | |||
! scope="col" width="100" | μL of 1 M NaOH<sub>stock</sub> | |||
! scope="col" width="100" | μL of AuCl<sub>3stock</sub> | |||
! scope="col" width="100" | μL of Fructose<sub>stock</sub> | |||
! scope="col" width="100" | μL of BSA<sub>stock</sub> | |||
! scope="col" width="100" | μL of Water | |||
|- | |||
| 4 || 500 || 0 || 0 || 335 || 422 || 316 || 3427 | |||
|- | |||
| 5 || 50 || 0 || 0 || 335 || 422 || 316 || 3877 | |||
|- | |||
| 6 || 5 || 0 || 0 || 335 || 422 || 316 || 3922 | |||
|- | |||
| 7 || 0 || 0 || 0 || 335 || 422 || 316 || 3927 | |||
|- | |||
| 8 || 0 || 5 || 0 || 335 || 422 || 316 || 3922 | |||
|- | |||
| 9 || 0 || 50 || 0 || 335 || 422 || 316 || 3877 | |||
|- | |||
| 10 || 0 || 500 || 0 || 335 || 422 || 316 || 3427 | |||
|- | |||
| 11 || 0 || 0 || 5 || 335 || 422 || 316 || 3922 | |||
|- | |||
| 12 || 0 || 0 || 50 || 335 || 422 || 316 || 3877 | |||
|} | |||
==Objective== | ==Objective== |
Revision as of 09:49, 21 September 2016
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
UV Vis round 3pH 4 - 12 Fructose concentration : 0.74 mM, 1.25 mM BSA stock solutions go 4 hours in oven, up to 80 C. menu > hill icon > menu > choose program 1 > 1 loop Solutions
ObjectiveDraw conclusions on the concentration of gold nano particles formed, and how the amount formed depends on 1) pH and 2) Fructose concentration. Concentration of nano particles is directly related to absorbance at the relevant wavelength. Protocol
Data AnalysisNotes |