User:Sarah Burkhard/Notebook/471 Nano Notebook/2016/11/16: Difference between revisions

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==Notes==
==Notes==
gold may absorb at 300, protein at 280
gold may absorb at 300, protein at 280
what if we plotted absorbance at 530 vs time and emission at 420 vs time on same graph ?


==Presentation notes==
==Presentation notes==

Latest revision as of 02:09, 27 September 2017

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Ocean Optics pH 11

Sample preparation:


Data Analysis

This chart shows the maximum absorbance over time.

This chart shows the maximum wavelength over time.

This chart shows the A(513.15) over time for pH 11 gold nanoparticles.


Notes

gold may absorb at 300, protein at 280

what if we plotted absorbance at 530 vs time and emission at 420 vs time on same graph ?

Presentation notes

  • Data analysis done by Cassidy and Sara -- Myoglobin

no NP at ph 11 and 12 know PI of protein identify all peaks area under curve vs time vs wavelength Gaussian fits for single pH and single time protein unfolds and shifts. no quenching gold 3+ and emission of Tryptophan. protonated state of lysine necessary for reduction. at neutral (11) no NP formation bc lysine deprotonated. fluorescence at pH 11: intensity of fluorescence : stopped. dip: unfolding, tryptophan environment sensitive. exposing T more, quenching it more.

silver NP: pH 11 and 12. cloudiness caused by : scattered . big particles. larger particles in general. broad peak: large distribution of sizes.

think about diff fluorescence might be. what dynamics are consistent with data from UV . back up claims w literature references. may be Nitrogens, not Tryptophan and other aromatic residues.



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