User:Sarah Burkhard/Notebook/471 Nano Notebook/2017/03/21: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Objectives== | ==Objectives== | ||
* UV-Vis prepared solutions | * UV-Vis prepared solutions | ||
* Prepare ~ 50mM His solution | |||
[[Image:Screenshot 2017-03-21 at 2.03.24 PM.png]] | |||
==Observations== | ==Observations== | ||
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* The Cys-Arg mix absorbs at a wavelength around 370 nm, and shows a shoulder around 240 nm. | * The Cys-Arg mix absorbs at a wavelength around 370 nm, and shows a shoulder around 240 nm. | ||
* The capped pH | * The capped pH absorbs at 290 nm. | ||
==Discussion== | ==Discussion== | ||
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We will run Histidine at pH 7 , 10, 12, and 14 | '''We will run Histidine at pH 4, 7 , 10, 12, and 14 .''' | ||
'''Make graph of Arg absorbance at different pH''' | |||
Latest revision as of 02:16, 27 September 2017
Project name | Main project page Previous entry Next entry |
Objectives
Observations
DiscussionThe yellow color from the capped Arg may come from a reaction of the N-cap, which has a carboxamide group. Yellow in specific often indicates amino groups (N=O) . This could be further confirmed using IR spectroscopy. Further, the fact that NPs formed above pK2, where the amino acid is negatively charged (above pH 13.2) , leads us to run further tests with Histidine, which has a lower pK2 and therefore a wider range of pH we can test. Histidine will have a negative one charge at pH above 8.97. pKa1 1.78 (carboxylic group will be protonated only at pH below 1.78 ) pKa2 8.97 (amine will be protonated only at pH values below 8.97 ) pKaR 5.97 (amine in R chain will be protonated at pH values below 5.97) charges: below 1.78: charge is 2+ 1.78 - 5.97 : +1 5.97 - 8.97 : 0 above 8.97 : -1
Make graph of Arg absorbance at different pH
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