User:Sarah Burkhard/Notebook/471 Nano Notebook/2017/03/21: Difference between revisions
From OpenWetWare
Line 27: | Line 27: | ||
pKa1 1.78 (carboxylic group will be protonated only at pH below 1.78 ) | pKa1 1.78 (carboxylic group will be protonated only at pH below 1.78 ) | ||
pKa2 8.97 (amine will be protonated only at pH values below 8.97 ) | pKa2 8.97 (amine will be protonated only at pH values below 8.97 ) | ||
pKaR 5.97 (amine in R chain will be protonated at pH values below 5.97) | pKaR 5.97 (amine in R chain will be protonated at pH values below 5.97) | ||
charges: | charges: | ||
below 1.78: charge is 2+ | below 1.78: charge is 2+ | ||
1.78 - 5.97 : +1 | 1.78 - 5.97 : +1 | ||
5.97 - 8.97 : 0 | 5.97 - 8.97 : 0 | ||
above 8.97 : -1 | above 8.97 : -1 | ||
Revision as of 10:42, 21 March 2017
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Objectives
Observations
DiscussionThe yellow color from the capped Arg may come from a reaction of the N-cap, which has a carboxamide group. Yellow in specific often indicates amino groups (N=O) . This could be further confirmed using IR spectroscopy. Histidine will have a negative one charge at pH above 8.97. pKa1 1.78 (carboxylic group will be protonated only at pH below 1.78 ) pKa2 8.97 (amine will be protonated only at pH values below 8.97 ) pKaR 5.97 (amine in R chain will be protonated at pH values below 5.97) charges: below 1.78: charge is 2+ 1.78 - 5.97 : +1 5.97 - 8.97 : 0 above 8.97 : -1
|