User:Saroj Pandey/Notebook/SNP PCR optimization/2014/09/18
 Project name
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SNP PCRPrimers ending in G
TAS2R38_1f: GGGATGTAGTGAAGAGGCAGG Length: 21 bp Tm: 61.0 °C GC: 57.1 % ANY: 2.0 SELF: 0.0 TAS2R38_1r: GATGGCTTGGTAGCTGTGGT Length: 20 bp Tm: 60.1 °C GC: 55.0 % ANY: 4.0 SELF: 0.0 Product Size: 161 bp Pair Any: 3.0 Pair End: 0.0
TAS2R38_2f: GGTGGCAACCAGGTCTTTAG Length: 20 bp Tm: 59.6 °C GC: 55.0 % ANY: 6.0 SELF: 2.0 TAS2R38_2r: CAATCACTGTTGCTCAGTGC Length: 20 bp Tm: 58.0 °C GC: 50.0 % ANY: 6.0 SELF: 4.0 Product Size: 235 bp Pair Any: 5.0 Pair End: 1.0
Primers ending in C
TAS2R38_C1f: GGGATGTAGTGAAGAGGCAGC Length:21 bp Tm: 61.2 °C GC:57.1 % ANY:3.0 SELF:3.0 TAS2R38_C1r: GATGGCTTGGTAGCTGTGGT Length:20 bp Tm: 60.1 °C GC:55.0 % ANY:4.0 SELF:0.0
TAS2R38_C2f: GGTGGCAACCAGGTCTTTAG Length:20 bp Tm: 59.6 °C GC:55.0 % ANY:6.0 SELF:2.0 TAS2R38_C2f: CAATCACTGTTGCTCAGTGG Length:20 bp Tm: 57.8 °C GC:50.0 % ANY:6.0 SELF:4.0
PCR 1
  PCR 2
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