User:Sean E. Lauber
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=====Molecular Techniques===== | =====Molecular Techniques===== | ||
*[[Sean_Lauber:Cytokeratin IHC staining| Cytokeratin IHC staining]] | *[[Sean_Lauber:Cytokeratin IHC staining| Cytokeratin IHC staining]] | ||
| - | *[[ | + | *[[Sean_Lauber:ELISA Development| ELISA Development]] |
| - | *[[ | + | *[[Sean_Lauber:Stable Transfection| Stable Transfection]] |
| - | *[[ | + | *[[Sean_Lauber:qRT-PCR (TaqMan)| qRT-PCR (TaqMan)]] |
| - | *[[ | + | *[[Sean_Lauber:RNA Extraction Using Trizol| RNA Extraction Using Trizol]] |
| - | *[[ | + | *[[Sean_Lauber:DNA Extraction Using Trizol| DNA Extraction Using Trizol]] |
=====Mammalian Cell Culture===== | =====Mammalian Cell Culture===== | ||
| - | *[[ | + | *[[Sean_Lauber:Freezing Mammalian Cells| Freezing Mammalian Cells]] |
| - | *[[ | + | *[[Sean_Lauber:Thawing Mammalian Cells| Thawing Mammalian Cells]] |
| - | *[[ | + | *[[Sean_Lauber:Culturing Lewis Lung Carcinoma Cells| Culturing Lewis Lung Carcinoma Cells]] |
| - | *[[ | + | *[[Sean_Lauber:MTT Cell Proliferation Assay| MTT Cell Proliferation Assay]] |
=====Technical===== | =====Technical===== | ||
| - | *[[ | + | *[[Sean_Lauber:Agilent Bioanalyzer| Agilent Bioanalyzer]] |
| - | *[[ | + | *[[Sean_Lauber:Statistics in Graphpad| Statistics in Graphpad]] |
| - | *[[ | + | *[[Sean_Lauber:AMIRA - Creating Label Fields by Lung Segmentation| AMIRA - Creating Label Fields by Lung Segmentation]] |
| - | *[[ | + | *[[Sean_Lauber:AMIRA - Generating Histograms| AMIRA - Generating Histograms]] |
| - | *[[ | + | *[[Sean_Lauber:AMIRA - Density Overlays| AMIRA - Density Overlays]] |
| - | *[[ | + | *[[Sean_Lauber:ImageJ - Threshold Analysis| ImageJ - Threshold Analysis]] |
| - | *[[ | + | *[[Sean_Lauber:Using the 7900 HT qPCR Machine| Using the 7900 HT qPCR Machine]] |
=====Animal Work===== | =====Animal Work===== | ||
| - | *[[ | + | *[[Sean_Lauber:Broncho-alveolar Lavage| Broncho-alveolar Lavage]] |
| - | *[[ | + | *[[Sean_Lauber:Peritoneal Lavage| Peritoneal Lavage]] |
| - | *[[ | + | *[[Sean_Lauber:Formalin Fixation & Preparation for Histology| Formalin Fixation & Preparation for Histology]] |
| - | *[[ | + | *[[Sean_Lauber:Mouse Endotracheal Intubation| Mouse Endotracheal Intubation]] |
| - | *[[ | + | *[[Sean_Lauber:CaPi Precipitation of Adenovirus| CaPi Precipitation of Adenovirus]] |
| - | *[[ | + | *[[Sean_Lauber:Kras G12D Genotyping| Kras G12D Genotyping]] |
==Links== | ==Links== | ||
Revision as of 14:11, 29 March 2012
Contents |
Contact Info
- sean.laub@gmail.com
Education
- M.Sc Candidate, Medical Sciences (Immunology), McMaster University, Hamilton, ON 2010-2012
- B.Sc, Honors Cell & Molecular Biology, Concordia University, Montreal, QC 2005-2010
- DEC, Natural Sciences, John Abbott College, Montreal, QC 2002-2004
Research interests
Protocols
Joyce Lab Protocols
- DNA Electrophoresis
- Bacterial Transformation
- T-tailing pBSKS
- Ethanol precipitation of DNA
- DNA Phenol extraction
- Preparing RNA from yeast
- Extraction of DNA from an agarose gel
- DNA Ligation
Richards Lab Protocols
Molecular Techniques
- Cytokeratin IHC staining
- ELISA Development
- Stable Transfection
- qRT-PCR (TaqMan)
- RNA Extraction Using Trizol
- DNA Extraction Using Trizol
Mammalian Cell Culture
- Freezing Mammalian Cells
- Thawing Mammalian Cells
- Culturing Lewis Lung Carcinoma Cells
- MTT Cell Proliferation Assay
Technical
- Agilent Bioanalyzer
- Statistics in Graphpad
- AMIRA - Creating Label Fields by Lung Segmentation
- AMIRA - Generating Histograms
- AMIRA - Density Overlays
- ImageJ - Threshold Analysis
- Using the 7900 HT qPCR Machine
Animal Work
- Broncho-alveolar Lavage
- Peritoneal Lavage
- Formalin Fixation & Preparation for Histology
- Mouse Endotracheal Intubation
- CaPi Precipitation of Adenovirus
- Kras G12D Genotyping
Links
Protocols and general information
- Recipes and protocols for stock solutions, competent cells, mini/midi/maxi prep and more
- Protocol-online Protocols
- Biowww.net Protocols
- Restriction digests
- Restriction digest troubleshooting
- More troubleshooting
- Journal of Visualized Experiments
- PSORT
Useful links
- Bitesize Bio
- BioForum
- Scientist Solutions
- Biochemweb.org
- E. coli Genotypes
- Online Mendelian Inheritance in Man
- NCBI
- Uniprot (as good as NCBI)
- Nebcutter 2.0
- Translate DNA
- Promoter Predictor
- Download the Molecular Biology Toolbar
- Backup your data with Mozy
