User:Sean E. Lauber: Difference between revisions

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==Education==
==Education==
*M.Sc Candidate, Medical Sciences (Immunology), McMaster University, Hamilton, ON 2010-2012
*M.Sc, Medical Sciences (Immunology), McMaster University, Hamilton, ON 2010-2012
*B.Sc, Honors Cell & Molecular Biology, Concordia University, Montreal, QC 2005-2010
*B.Sc, Honors Cell & Molecular Biology, Concordia University, Montreal, QC 2005-2010
*DEC, Natural Sciences, John Abbott College, Montreal, QC 2002-2004
*DEC, Natural Sciences, John Abbott College, Montreal, QC 2002-2004


==Research interests==
==Protocols==
 
====Richards Lab Protocols====


=====Molecular Techniques=====
*[[Sean_Lauber:Cytokeratin IHC staining| Cytokeratin IHC staining]]
*[[Sean_Lauber:ELISA Development| ELISA Development]]
*[[Sean_Lauber:Isotype ELISA against Adenovirus | Isotype ELISA against Adenovirus]]
*[[Sean_Lauber:Generating Adenovirus Lysate | Generating Adenovirus Lysate]]
*[[Sean_Lauber:PCR| PCR]]
*[[Sean_Lauber:Stable Transfection Using Lipofectamine 2000| Stable Transfection Using Lipofectamine 2000]]
*[[Sean_Lauber:RNA Extraction Using Trizol| RNA Extraction Using Trizol]]
*[[Sean_Lauber:DNA Extraction Using Trizol| DNA Extraction Using Trizol]]
*[[Sean_Lauber:DNase Treatment Using DNA-Free| DNase Treatment Using DNA-Free (for Taqman)]]
*[[Sean_Lauber:Reverse Transcription using Superscript II| Reverse Transcription using Superscript II (for Taqman)]]
*[[Sean_Lauber:qRT-PCR (TaqMan)| qRT-PCR (TaqMan)]]


=====Mammalian Cell Culture=====
*[[Sean_Lauber:Freezing Mammalian Cells| Freezing Mammalian Cells]]
*[[Sean_Lauber:Thawing Mammalian Cells| Thawing Mammalian Cells]]
*[[Sean_Lauber:Culturing Lewis Lung Carcinoma Cells| Culturing Lewis Lung Carcinoma Cells]]
*[[Sean_Lauber:MTT Cell Proliferation Assay| MTT Cell Proliferation Assay]]
*[[Sean_Lauber:Cytokine Stimulation of Cells| Cytokine Stimulation of Cells]]
*[[Sean_Lauber:Culturing of Primary Macrophages| Culturing of Primary Macrophages]]


==Protocols==
=====Technical=====
*[[Sean_Lauber:Agilent Bioanalyzer| Agilent Bioanalyzer]]
*[[Sean_Lauber:Statistics in Graphpad| Statistics in Graphpad]]
*[[Sean_Lauber:AMIRA - Creating Label Fields by Lung Segmentation| AMIRA - Creating Label Fields by Lung Segmentation]]
*[[Sean_Lauber:AMIRA - Generating Histograms| AMIRA - Generating Histograms]]
*[[Sean_Lauber:AMIRA - Density Overlays| AMIRA - Density Overlays]]
*[[Sean_Lauber:ImageJ - Threshold Analysis| ImageJ - Threshold Analysis]]
*[[Sean_Lauber:Using the 7900 HT qPCR Machine| Using the 7900 HT qPCR Machine]]
*[[Sean_Lauber:Heat maps in Excel| Heat maps in Excel]]
 
=====Animal Work=====
*[[Sean_Lauber:Broncho-alveolar Lavage| Broncho-alveolar Lavage]]
*[[Sean_Lauber:Peritoneal Lavage| Peritoneal Lavage]]
*[[Sean_Lauber:Formalin Fixation & Preparation for Histology| Formalin Fixation & Preparation for Histology]]
*[[Sean_Lauber:Mouse Endotracheal Intubation| Mouse Endotracheal Intubation]]
*[[Sean_Lauber:Using the Kras G12D Mouse Model for Lung Cancer| Using the Kras G12D Mouse Model for Lung Cancer]]
*[[Sean_Lauber:Using the Lewis Lung Carcinoma cell Mouse Model for Lung Cancer| Using the Lewis Lung Carcinoma cell Mouse Model for Lung Cancer]]
*[[Sean_Lauber:Kras G12D Genotyping| Kras G12D Genotyping]]
*[[Sean_Lauber:Differential cell staining|Differential cell staining]]


====Joyce Lab Protocols====   
====Joyce Lab Protocols====   
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*[[Joyce:DNA electrophoresis| DNA Electrophoresis]]
*[[Joyce:DNA electrophoresis| DNA Electrophoresis]]
*[[Joyce:Transformation| Transformation]]
*[[Joyce:Transformation| Bacterial Transformation]]
*[[Joyce: T-tailing pBSKS| T-tailing pBSKS]]
*[[Joyce: T-tailing pBSKS| T-tailing pBSKS]]
*[[Joyce: DNA precipitation| Ethanol precipitation of DNA]]
*[[Joyce: DNA precipitation| Ethanol precipitation of DNA]]
*[[Joyce: Phenol extraction| Phenol extraction]]
*[[Joyce: Phenol extraction| DNA Phenol extraction]]
*[[Joyce: Preparing RNA from yeast|Preparing RNA from yeast]]
*[[Joyce: Preparing RNA from yeast|Preparing RNA from yeast]]
*[[Joyce: Extraction of DNA from an agarose gel|Extraction of DNA from an agarose gel]]
*[[Joyce: Extraction of DNA from an agarose gel|Extraction of DNA from an agarose gel]]
*[[Joyce: Ligation|Ligation]]
*[[Joyce: Ligation| DNA Ligation]]
 
*[[Sean_Lauber: PCR Mutagenesis| PCR Mutagenesis]]
 
====Richards Lab Protocols====
 
=====Molecular Techniques=====
*[[Richards:Cytokeratin IHC staining| Cytokeratin IHC staining]]
*[[Richards:ELISA Development| ELISA Development]]
*[[Richards:MTT Cell Proliferation Assay| MTT Cell Proliferation Assay]]
*[[Richards:Stable Transfection| Stable Transfection]]
*[[Richards:qRT-PCR (TaqMan)| qRT-PCR (TaqMan)]]
*[[Richards:RNA Extraction Using Trizol| RNA Extraction Using Trizol]]
 
=====Mammalian Cell Culture=====
*[[Richards:Freezing Mammalian Cells| Freezing Mammalian Cells]]
*[[Richards:Thawing Mammalian Cells| Thawing Mammalian Cells]]
*[[Richards:Culturing Lewis Lung Carcinoma Cells| Culturing Lewis Lung Carcinoma Cells]]
 
=====Technical=====
*[[Richards:Agilent Bioanalyzer| Agilent Bioanalyzer]]
 
=====Animal Work=====
*[[Richards:Broncho-alveolar Lavage| Broncho-alveolar Lavage]]
*[[Richards:Peritoneal Lavage| Peritoneal Lavage]]
*[[Richards:Mouse Endotracheal Intubation| Mouse Endotracheal Intubation]]
*[[Richards:CaPi Precipitation of Adenovirus| CaPi Precipitation of Adenovirus]]
*[[Richards:Kras G12D Genotyping| Kras G12D Genotyping]]


==Links==
==Links==
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*[http://psort.ims.u-tokyo.ac.jp/ PSORT]
*[http://psort.ims.u-tokyo.ac.jp/ PSORT]


====Fun stuff, useful links====
====Useful links====


*[http://bitesizebio.com/ Bitesize Bio]
*[http://bitesizebio.com/ Bitesize Bio]
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*[http://molecularbiology.toolbar.fm/ Download the Molecular Biology Toolbar]
*[http://molecularbiology.toolbar.fm/ Download the Molecular Biology Toolbar]
*[https://mozy.com/home Backup your data with Mozy]
*[https://mozy.com/home Backup your data with Mozy]
*[http://www.abcam.com/index.html?pageconfig=resource&rid=11384 Useful IHC optimization tips for paraffin embedded tissue (Abcam)]


====Openwetware related====
====Openwetware related====
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*[[OpenWetWare:Welcome|Introductory tutorial]]
*[[OpenWetWare:Welcome|Introductory tutorial]]
*[[Help|OpenWetWare help pages]]
*[[Help|OpenWetWare help pages]]
*[[Notebook|My Notebook]]


====Technical guides====


*[[Lauber: I won't forget|I won't forget a thing]]
*[http://www.dako.com/08002_ihc_staining_methods_5ed.pdf Dako's IHC Guide]

Revision as of 08:58, 6 November 2012

Contact Info

  • sean.laub@gmail.com

Education

  • M.Sc, Medical Sciences (Immunology), McMaster University, Hamilton, ON 2010-2012
  • B.Sc, Honors Cell & Molecular Biology, Concordia University, Montreal, QC 2005-2010
  • DEC, Natural Sciences, John Abbott College, Montreal, QC 2002-2004

Protocols

Richards Lab Protocols

Molecular Techniques
Mammalian Cell Culture
Technical
Animal Work

Joyce Lab Protocols

Links

Protocols and general information

Useful links

Openwetware related

Technical guides

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