User:Sean E. Lauber: Difference between revisions
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==Education== | ==Education== | ||
*M.Sc | *M.Sc, Medical Sciences (Immunology), McMaster University, Hamilton, ON 2010-2012 | ||
*B.Sc, Honors Cell & Molecular Biology, Concordia University, Montreal, QC 2005-2010 | *B.Sc, Honors Cell & Molecular Biology, Concordia University, Montreal, QC 2005-2010 | ||
*DEC, Natural Sciences, John Abbott College, Montreal, QC 2002-2004 | *DEC, Natural Sciences, John Abbott College, Montreal, QC 2002-2004 | ||
== | ==Protocols== | ||
====Richards Lab Protocols==== | |||
=====Molecular Techniques===== | |||
*[[Sean_Lauber:Cytokeratin IHC staining| Cytokeratin IHC staining]] | |||
*[[Sean_Lauber:ELISA Development| ELISA Development]] | |||
*[[Sean_Lauber:Isotype ELISA against Adenovirus | Isotype ELISA against Adenovirus]] | |||
*[[Sean_Lauber:Generating Adenovirus Lysate | Generating Adenovirus Lysate]] | |||
*[[Sean_Lauber:PCR| PCR]] | |||
*[[Sean_Lauber:RNA Extraction Using Trizol| RNA Extraction Using Trizol]] | |||
*[[Sean_Lauber:DNA Extraction Using Trizol| DNA Extraction Using Trizol]] | |||
*[[Sean_Lauber:DNase Treatment Using DNA-Free| DNase Treatment Using DNA-Free (for Taqman)]] | |||
*[[Sean_Lauber:Reverse Transcription using Superscript II| Reverse Transcription using Superscript II (for Taqman)]] | |||
*[[Sean_Lauber:qRT-PCR (TaqMan)| qRT-PCR (TaqMan)]] | |||
=====Mammalian Cell Culture===== | |||
*[[Sean_Lauber:Freezing Mammalian Cells| Freezing Mammalian Cells]] | |||
*[[Sean_Lauber:Thawing Mammalian Cells| Thawing Mammalian Cells]] | |||
*[[Sean_Lauber:Culturing Lewis Lung Carcinoma Cells| Culturing Lewis Lung Carcinoma Cells]] | |||
*[[Sean_Lauber:MTT Cell Proliferation Assay| MTT Cell Proliferation Assay]] | |||
*[[Sean_Lauber:Cytokine Stimulation of Cells| Cytokine Stimulation of Cells]] | |||
*[[Sean_Lauber:Culturing of Primary Macrophages| Culturing of Primary Macrophages]] | |||
*[[Sean_Lauber:Adenovirus infection (in vitro)| Adenovirus infection (in vitro)]] | |||
=====Technical===== | |||
*[[Sean_Lauber:Agilent Bioanalyzer| Agilent Bioanalyzer]] | |||
*[[Sean_Lauber:Statistics in Graphpad| Statistics in Graphpad]] | |||
*[[Sean_Lauber:AMIRA - Creating Label Fields by Lung Segmentation| AMIRA - Creating Label Fields by Lung Segmentation]] | |||
*[[Sean_Lauber:AMIRA - Generating Histograms| AMIRA - Generating Histograms]] | |||
*[[Sean_Lauber:AMIRA - Density Overlays| AMIRA - Density Overlays]] | |||
*[[Sean_Lauber:ImageJ - Threshold Analysis| ImageJ - Threshold Analysis]] | |||
*[[Sean_Lauber:Using the 7900 HT qPCR Machine| Using the 7900 HT qPCR Machine]] | |||
*[[Sean_Lauber:Heat maps in Excel| Heat maps in Excel]] | |||
== | =====Animal Work===== | ||
*[[Sean_Lauber:Broncho-alveolar Lavage| Broncho-alveolar Lavage]] | |||
*[[Sean_Lauber:Peritoneal Lavage| Peritoneal Lavage]] | |||
*[[Sean_Lauber:Formalin Fixation & Preparation for Histology| Formalin Fixation & Preparation for Histology]] | |||
*[[Sean_Lauber:Mouse Endotracheal Intubation| Mouse Endotracheal Intubation]] | |||
*[[Sean_Lauber:Using the Kras G12D Mouse Model for Lung Cancer| Using the Kras G12D Mouse Model for Lung Cancer]] | |||
*[[Sean_Lauber:Using the Lewis Lung Carcinoma cell Mouse Model for Lung Cancer| Using the Lewis Lung Carcinoma cell Mouse Model for Lung Cancer]] | |||
*[[Sean_Lauber:Kras G12D Genotyping| Kras G12D Genotyping]] | |||
*[[Sean_Lauber:Differential cell staining|Differential cell staining]] | |||
====Joyce Lab Protocols==== | ====Joyce Lab Protocols==== | ||
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*[[Joyce:DNA electrophoresis| DNA Electrophoresis]] | *[[Joyce:DNA electrophoresis| DNA Electrophoresis]] | ||
*[[Joyce:Transformation| Transformation]] | *[[Joyce:Transformation| Bacterial Transformation]] | ||
*[[Joyce: T-tailing pBSKS| T-tailing pBSKS]] | *[[Joyce: T-tailing pBSKS| T-tailing pBSKS]] | ||
*[[Joyce: DNA precipitation| Ethanol precipitation of DNA]] | *[[Joyce: DNA precipitation| Ethanol precipitation of DNA]] | ||
*[[Joyce: Phenol extraction| Phenol extraction]] | *[[Joyce: Phenol extraction| DNA Phenol extraction]] | ||
*[[Joyce: Preparing RNA from yeast|Preparing RNA from yeast]] | *[[Joyce: Preparing RNA from yeast|Preparing RNA from yeast]] | ||
*[[Joyce: Extraction of DNA from an agarose gel|Extraction of DNA from an agarose gel]] | *[[Joyce: Extraction of DNA from an agarose gel|Extraction of DNA from an agarose gel]] | ||
*[[Joyce: Ligation|Ligation | *[[Joyce: Ligation| DNA Ligation]] | ||
*[[Sean_Lauber: PCR Mutagenesis| PCR Mutagenesis]] | |||
*[[ | |||
==Links== | ==Links== | ||
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*[http://psort.ims.u-tokyo.ac.jp/ PSORT] | *[http://psort.ims.u-tokyo.ac.jp/ PSORT] | ||
==== | ====Useful links==== | ||
*[http://bitesizebio.com/ Bitesize Bio] | *[http://bitesizebio.com/ Bitesize Bio] | ||
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*[http://www.fruitfly.org/seq_tools/promoter.html Promoter Predictor] | *[http://www.fruitfly.org/seq_tools/promoter.html Promoter Predictor] | ||
*[http://molecularbiology.toolbar.fm/ Download the Molecular Biology Toolbar] | *[http://molecularbiology.toolbar.fm/ Download the Molecular Biology Toolbar] | ||
*[ | *[http://www.abcam.com/index.html?pageconfig=resource&rid=11384 Useful IHC optimization tips for paraffin embedded tissue (Abcam)] | ||
====Openwetware related==== | ====Openwetware related==== | ||
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*[[OpenWetWare:Welcome|Introductory tutorial]] | *[[OpenWetWare:Welcome|Introductory tutorial]] | ||
*[[Help|OpenWetWare help pages]] | *[[Help|OpenWetWare help pages]] | ||
====Technical guides==== | |||
*[ | *[http://www.dako.com/08002_ihc_staining_methods_5ed.pdf Dako's IHC Guide] |
Revision as of 11:28, 11 December 2012
Contact Info
- sean.laub@gmail.com
Education
- M.Sc, Medical Sciences (Immunology), McMaster University, Hamilton, ON 2010-2012
- B.Sc, Honors Cell & Molecular Biology, Concordia University, Montreal, QC 2005-2010
- DEC, Natural Sciences, John Abbott College, Montreal, QC 2002-2004
Protocols
Richards Lab Protocols
Molecular Techniques
- Cytokeratin IHC staining
- ELISA Development
- Isotype ELISA against Adenovirus
- Generating Adenovirus Lysate
- PCR
- RNA Extraction Using Trizol
- DNA Extraction Using Trizol
- DNase Treatment Using DNA-Free (for Taqman)
- Reverse Transcription using Superscript II (for Taqman)
- qRT-PCR (TaqMan)
Mammalian Cell Culture
- Freezing Mammalian Cells
- Thawing Mammalian Cells
- Culturing Lewis Lung Carcinoma Cells
- MTT Cell Proliferation Assay
- Cytokine Stimulation of Cells
- Culturing of Primary Macrophages
- Adenovirus infection (in vitro)
Technical
- Agilent Bioanalyzer
- Statistics in Graphpad
- AMIRA - Creating Label Fields by Lung Segmentation
- AMIRA - Generating Histograms
- AMIRA - Density Overlays
- ImageJ - Threshold Analysis
- Using the 7900 HT qPCR Machine
- Heat maps in Excel
Animal Work
- Broncho-alveolar Lavage
- Peritoneal Lavage
- Formalin Fixation & Preparation for Histology
- Mouse Endotracheal Intubation
- Using the Kras G12D Mouse Model for Lung Cancer
- Using the Lewis Lung Carcinoma cell Mouse Model for Lung Cancer
- Kras G12D Genotyping
- Differential cell staining
Joyce Lab Protocols
- DNA Electrophoresis
- Bacterial Transformation
- T-tailing pBSKS
- Ethanol precipitation of DNA
- DNA Phenol extraction
- Preparing RNA from yeast
- Extraction of DNA from an agarose gel
- DNA Ligation
- PCR Mutagenesis
Links
Protocols and general information
- Recipes and protocols for stock solutions, competent cells, mini/midi/maxi prep and more
- Protocol-online Protocols
- Biowww.net Protocols
- Restriction digests
- Restriction digest troubleshooting
- More troubleshooting
- Journal of Visualized Experiments
- PSORT
Useful links
- Bitesize Bio
- BioForum
- Scientist Solutions
- Biochemweb.org
- E. coli Genotypes
- Online Mendelian Inheritance in Man
- NCBI
- Uniprot (as good as NCBI)
- Nebcutter 2.0
- Translate DNA
- Promoter Predictor
- Download the Molecular Biology Toolbar
- Useful IHC optimization tips for paraffin embedded tissue (Abcam)